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Literature DB >> 7966638

Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.

Y Tanji¹, M Hijikata, Y Hirowatari, K Shimotohno.

Abstract

Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase and mutation analyses were performed by using a transient protein production system in cultured cells. Pulse-chase study revealed the sequential production of HCV-NS proteins. Production of p70(NS3) and p66(NS5B) were rapid. An 89-kDa processing intermediate protein (p89) was observed during the early part of the chase. p89 seemed to be cleaved first into a 31-kDa protein (p31) and a p58/56(NS5A). p31 was further processed into p4(NS4A) and p27(NS4B). Mutation analysis of cleavage sites of NS4A/4B, NS4B/5A, and NS5A/5B revealed that cleavage at each site is essentially independent from cleavage occurring at the other site.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1994 PMID： 7966638 PMCID： PMC237315 DOI： 10.1128/JVI.68.12.8418-8422.1994

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

21 in total

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Authors: H Okamoto; S Okada; Y Sugiyama; K Kurai; H Iizuka; A Machida; Y Miyakawa; M Mayumi
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3. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors: H Schägger; G von Jagow
Journal: Anal Biochem Date: 1987-11-01 Impact factor: 3.365

4. Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction.

Authors: N Kato; O Yokosuka; M Omata; K Hosoda; M Ohto
Journal: J Clin Invest Date: 1990-11 Impact factor: 14.808

5. Structure and organization of the hepatitis C virus genome isolated from human carriers.

Authors: A Takamizawa; C Mori; I Fuke; S Manabe; S Murakami; J Fujita; E Onishi; T Andoh; I Yoshida; H Okayama
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6. Genetic organization and diversity of the hepatitis C virus.

Authors: Q L Choo; K H Richman; J H Han; K Berger; C Lee; C Dong; C Gallegos; D Coit; R Medina-Selby; P J Barr
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7. Processing of hepatitis C viral polyprotein in Escherichia coli.

Authors: Y Komoda; M Hijikata; Y Tanji; Y Hirowatari; H Mizushima; K Kimura; K Shimotohno
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8. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.

Authors: G Kuo; Q L Choo; H J Alter; G L Gitnick; A G Redeker; R H Purcell; T Miyamura; J L Dienstag; M J Alter; C E Stevens
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9. Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes.

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10. The Taiwanese hepatitis C virus genome: sequence determination and mapping the 5' termini of viral genomic and antigenomic RNA.

Authors: P J Chen; M H Lin; K F Tai; P C Liu; C J Lin; D S Chen
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Authors: A Y Howe; R Chase; S S Taremi; C Risano; B Beyer; B Malcolm; J Y Lau
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6. Hepatitis C virus subgenomic replicons induce endoplasmic reticulum stress activating an intracellular signaling pathway.

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10. Increasing rate of cleavage at boundary between non-structural proteins 4B and 5A inhibits replication of hepatitis C virus.

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