Pregnancy after intracytoplasmic sperm injection of metaphase II oocytes with spermatozoa from a man with complete retrograde ejaculation.

Retrograde ejaculation is an uncommon cause of infertility, which has been treated successfully with different kinds of artificial reproduction technique, e.g. cervical cap artificial insemination by husband, intra-uterine and intraperitoneal insemination, standard in-vitro fertilization, pronuclear stage transfer and gamete intra-Fallopian transfer. All these techniques require a minimal number and motility of spermatozoa obtained after post-masturbation voiding. In some cases, only very few spermatozoa with very poor or no motility are found in the urine voided immediately after masturbation. In such a case, where no more than 14 spermatozoa were recovered over a 3 h search, intracytoplasmic sperm injection of metaphase II oocytes led to the development and replacement of three fair embryos, resulting in an ongoing twin pregnancy. This technique opens up perspectives for the treatment of men with complete retrograde ejaculation and quasi-azoospermic post-voiding specimens.