Literature DB >> 7959020

Versatile transformation vectors to assay the promoter activity of DNA elements in plants.

G Pasquali1, P B Ouwerkerk, J Memelink.   

Abstract

A convenient vector system was developed to evaluate transcriptional promoter activities in plants. Two primary vectors, optionally containing the cauliflower mosaic virus (CaMV) 35S -47 or -90 minimal promoters, offer multiple sites for cloning the sequence of interest upstream from the beta-glucuronidase gene (gusA). The promoter-gusA cassette can be transferred to a binary vector containing the selectable neomycin phosphotransferase II-encoding gene (nptII) next to the left border. In addition, the transferred DNA (T-DNA) contains the chloramphenicol acetyltransferase gene (cat) driven by the CaMV 35S promoter. Activity of cat can serve as a reference for gusA expression to correct for effect of chromosomal position or T-DNA copy number.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7959020     DOI： 10.1016/0378-1119(94)90179-1

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  15 in total

1.  Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice.

Authors:  Yi Chen; Aijun Sun; Mei Wang; Zhen Zhu; Pieter B F Ouwerkerk
Journal:  Plant Mol Biol       Date:  2013-11-27       Impact factor: 4.076

2.  The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds.

Authors:  S de Pater; K Pham; I Klitsie; J Kijne
Journal:  Plant Mol Biol       Date:  1996-11       Impact factor: 4.076

3.  Rice ASR1 and ASR5 are complementary transcription factors regulating aluminium responsive genes.

Authors:  Rafael Augusto Arenhart; Mariana Schunemann; Lauro Bucker Neto; Rogerio Margis; Zhi-Yong Wang; Marcia Margis-Pinheiro
Journal:  Plant Cell Environ       Date:  2015-12-14       Impact factor: 7.228

4.  Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment.

Authors:  L van der Fits; J Memelink
Journal:  Plant Mol Biol       Date:  1997-03       Impact factor: 4.076

5.  The promoter of the strictosidine synthase gene from periwinkle confers elicitor-inducible expression in transgenic tobacco and binds nuclear factors GT-1 and GBF.

Authors:  G Pasquali; A S Erven; P B Ouwerkerk; F L Menke; J Memelink
Journal:  Plant Mol Biol       Date:  1999-04       Impact factor: 4.076

6.  A Catharanthus roseus BPF-1 homologue interacts with an elicitor-responsive region of the secondary metabolite biosynthetic gene Str and is induced by elicitor via a JA-independent signal transduction pathway.

Authors:  L van der Fits; H Zhang; F L Menke; M Deneka; J Memelink
Journal:  Plant Mol Biol       Date:  2000-11       Impact factor: 4.076

7.  Elicitor-responsive promoter regions in the tryptophan decarboxylase gene from Catharanthus roseus.

Authors:  P B Ouwerkerk; J Memelink
Journal:  Plant Mol Biol       Date:  1999-01       Impact factor: 4.076

8.  Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthetic genes in catharanthus roseus

Authors: 
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

9.  Characterization of a zinc-dependent transcriptional activator from Arabidopsis.

Authors:  S de Pater; V Greco; K Pham; J Memelink; J Kijne
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

10.  Cloning and characterization of PR5 gene from Curcuma amada and Zingiber officinale in response to Ralstonia solanacearum infection.

Authors:  D Prasath; I El-Sharkawy; S Sherif; K S Tiwary; S Jayasankar
Journal:  Plant Cell Rep       Date:  2011-05-19       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.