Literature DB >> 7959014

Rapid isolation of highly productive recombinant Chinese hamster ovary cell lines.

B Miloux1, J H Lupker.   

Abstract

An expression system combining a unit for the expression of the gene of interest reinforced by the hepatitis B virus X transactivator and a selectable gene weakened by the insertion of an A+T-rich sequence derived from the 3'-untranslated region of the granulocyte-macrophage colony-stimulating factor mRNA is described. This vector allows rapid one-step isolation of highly productive Chinese hamster ovary clones.

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Year:  1994        PMID： 7959014     DOI： 10.1016/0378-1119(94)90173-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  4 in total

1.  A regulatable selective system facilitates isolation of heterologous protein hyper-producing mammalian cells without gene amplification.

Authors:  Kiichiro Teruya; Yingpei Zhang; Yoshinori Katakura; Sanetaka Shirahata
Journal:  Cytotechnology       Date:  2002-11       Impact factor: 2.058

2.  The active metabolite of Clopidogrel disrupts P2Y12 receptor oligomers and partitions them out of lipid rafts.

Authors:  Pierre Savi; Jean-Luc Zachayus; Nathalie Delesque-Touchard; Catherine Labouret; Caroline Hervé; Marie-Françoise Uzabiaga; Jean-Marie Pereillo; Jean-Michel Culouscou; Françoise Bono; Pascual Ferrara; Jean-Marc Herbert
Journal:  Proc Natl Acad Sci U S A       Date:  2006-07-11       Impact factor: 11.205

3.  Activation of mitogen-activated protein kinases by stimulation of the central cannabinoid receptor CB1.

Authors:  M Bouaboula; C Poinot-Chazel; B Bourrié; X Canat; B Calandra; M Rinaldi-Carmona; G Le Fur; P Casellas
Journal:  Biochem J       Date:  1995-12-01       Impact factor: 3.857

4.  Activation of mitogen-activated protein kinase couples neurotensin receptor stimulation to induction of the primary response gene Krox-24.

Authors:  C Poinot-Chazel; M Portier; M Bouaboula; N Vita; F Pecceu; D Gully; J G Monroe; J P Maffrand; G Le Fur; P Casellas
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857
  4 in total

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