Cloning of the rat edg-1 immediate-early gene: expression pattern suggests diverse functions.

The edg-1 immediate-early gene encodes a G-protein-coupled receptor homolog implicated in endothelial cell differentiation. We report the cloning of the rat edg-1 gene. Our Northern analyses indicate that edg-1 is much more widely expressed than previously thought. edg-1 mRNA was found in many organs at several stages of development with relatively high levels present in adult brain. edg-1 transcripts were also detected in several cell lines. Expression of edg-1 mRNA in the PC12 cell model of neuronal differentiation was unaffected by agents that cause PC12 cells to differentiate or proliferate. Therefore, edg-1 may play a cell-type-specific role in differentiation and also participate in neurotransmission.