OBJECTIVE: To test the effects of steroid-containing media such as follicular fluid (FF) and serum and of steroid-free but human protein-rich medium on hyperactivation (HA) of human spermatozoa with and without a preincubation period. DESIGN: Analysis of HA using a computer-aided sperm analysis system. SETTING: Academic hospital and andrologic laboratory. PATIENTS: Twenty-six sperm samples were obtained from 26 different IVF patients. INTERVENTIONS: None. RESULTS: After a preincubation period of 3 hours, FF and serum enhanced the HA after 1 hour. A human plasma protein-rich solution did not. Without preincubation, no stimulation of HA was observed. CONCLUSION: Steroids may be involved in HA, and a preincubation period may be required for an appropriate FF-induced HA.
OBJECTIVE: To test the effects of steroid-containing media such as follicular fluid (FF) and serum and of steroid-free but human protein-rich medium on hyperactivation (HA) of human spermatozoa with and without a preincubation period. DESIGN: Analysis of HA using a computer-aided sperm analysis system. SETTING: Academic hospital and andrologic laboratory. PATIENTS: Twenty-six sperm samples were obtained from 26 different IVFpatients. INTERVENTIONS: None. RESULTS: After a preincubation period of 3 hours, FF and serum enhanced the HA after 1 hour. A human plasma protein-rich solution did not. Without preincubation, no stimulation of HA was observed. CONCLUSION:Steroids may be involved in HA, and a preincubation period may be required for an appropriate FF-induced HA.
Authors: Aleksandra Łukasiewicz; Kari Huhta; Jarmo Ritari; Juha Peräsaari; Pia Allinen; Marjo Malinen; Annalaura Jokiniemi; Tanja Turunen; Jukka Partanen; Jukka Kekäläinen Journal: Int J Mol Sci Date: 2022-03-22 Impact factor: 5.923
Authors: Annalaura Jokiniemi; Liisa Kuusipalo; Jarmo Ritari; Satu Koskela; Jukka Partanen; Jukka Kekäläinen Journal: Heredity (Edinb) Date: 2020-08-03 Impact factor: 3.821