Literature DB >> 795581

A micromethod for stimulation of chicken lymphocytes in vitro using whole blood.

O Lassila, J Eskola, P Toivanen.   

Abstract

A simple, rapid and reproducible micromethod for determination of in vitro mitogenic responses of chicken peripheral blood lymphocytes is described. The test utilizes 5 mul volumes of heparinized whole blood, 125I-labelled 5-iodo-2'-deoxyuridine with 5-fluoro-2'-deoxyuridine in place of [3H]thymidine and a multiple cell-culture harvester. The method permits a close follow-up of the mitogenic responses in the chicken.

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Year:  1976        PMID： 795581      PMCID： PMC1540987     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  19 in total

Review 1.  Thymus-independent B-cell induction and paralysis.

Authors:  A Coutinho; G Möller
Journal:  Adv Immunol       Date:  1975       Impact factor: 3.543

2.  Selective activation of chicken T lymphocytes by concanavalin A.

Authors:  P Toivanen; A Toivanen
Journal:  J Immunol       Date:  1973-11       Impact factor: 5.422

3.  Whole-blood culture technique for functional studies of lymphocyte reactivity to mitogens, antigens, and homologous lymphocytes.

Authors:  J L Pauly; J E Sokal; T Han
Journal:  J Lab Clin Med       Date:  1973-09

Review 4.  Reactivation of chick erythrocyte nuclei by somatic cell hybridization.

Authors:  N R Ringertz; L Bolund
Journal:  Int Rev Exp Pathol       Date:  1974

5.  In vitro assay of mitogen stimulation of avian peripheral lymphocytes.

Authors:  L F Lee
Journal:  Avian Dis       Date:  1974 Oct-Dec       Impact factor: 1.577

Review 6.  Phytohemagglutin and concanavalin A: probes for murine 'T' cell activivation and differentiation.

Authors:  J D Stobo
Journal:  Transplant Rev       Date:  1972

7.  Potentation by fluorodeoxyuridine of 125I-deoxyuridine uptake by human and chicken lymphocytes in the quantitation of mitogenic response.

Authors:  T Asantila; P Toivanen
Journal:  J Immunol Methods       Date:  1974-12       Impact factor: 2.303

8.  A micromethod for lymphoblastic transformation of mouse lymphocytes from peripheral blood.

Authors:  H J Heiniger; J M Wolf; H W Chen; H Meier
Journal:  Proc Soc Exp Biol Med       Date:  1973-05

9.  Lymphocyte transformation using whole blood cultures: an analysis of responses.

Authors:  D W Paty; D Hughes
Journal:  J Immunol Methods       Date:  1972-11       Impact factor: 2.303

10.  Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals.

Authors:  T Han; J Pauly
Journal:  Clin Exp Immunol       Date:  1972-05       Impact factor: 4.330
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  3 in total

1.  In vivo effect of levamisole on cellular and humoral immunity in normal chickens.

Authors:  E Soppi; O Lassila; M K Viljanen; O P Lehtonen; J Eskola
Journal:  Clin Exp Immunol       Date:  1979-12       Impact factor: 4.330

2.  Non-specific effects of avian retrovirus co-incubation on lymphocyte function: abrogation of antigen- and mitogen-induced proliferative responsiveness.

Authors:  E Israel; M Yu; M A Wainberg
Journal:  Immunology       Date:  1979-09       Impact factor: 7.397

3.  L-Arginine stimulates intestinal intraepithelial lymphocyte functions and immune response in chickens orally immunized with live intermediate plus strain of infectious bursal disease vaccine.

Authors:  Chandrakant Tayade; Madhuri Koti; S C Mishra
Journal:  Vaccine       Date:  2006-04-18       Impact factor: 3.641
  3 in total

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