Literature DB >> 795548

Identification and location of the histone H2A and H3 genes by sequence analysis of sea urchin (S. purpuratus) DNA cloned in E. coli.

I Sures, A Maxam, R H Cohn, L H Kedes.   

Abstract

A 2000 base pair (bp) DNA fragment can be excised from sea urchin (S. purpuratus) histone gene repeat units with restriction endonuclease Eco R1. This DNA, which has been cloned in a bacterial plasmid, is known to encompass two of the five histone genes. The fragment has a single endonuclease Hind III cleavage site in one of the genes and a Hae III cleavage site in the other gene. We now report the nucleotide sequences of 62 bp adjacent to the Hind III site and 42 bp adjacent to the Hae III cleavage site. The results identify the cloned DNA as histone genes, show that it codes for histone proteins H2A and H3, and locate and orient H2A and H3 genes with respect to restriction endonuclease sites in the repeat unit.

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Year:  1976        PMID： 795548     DOI： 10.1016/0092-8674(76)90031-3

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  10 in total

1.  Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression.

Authors:  A Mauron; S Levy; G Childs; L Kedes
Journal:  Mol Cell Biol       Date:  1981-07       Impact factor: 4.272

2.  Differential patterns of transcript accumulation during human myogenesis.

Authors:  P Gunning; E Hardeman; R Wade; P Ponte; W Bains; H M Blau; L Kedes
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

Review 3.  Molecular cloning of DNA. An introduction into techniques and problems.

Authors:  H P Vosberg
Journal:  Hum Genet       Date:  1977-12-29       Impact factor: 4.132

4.  Histone genes of Volvox carteri: DNA sequence and organization of two H3-H4 gene loci.

Authors:  K Müller; R Schmitt
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

5.  Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5' region of H5 mRNA.

Authors:  R Ruiz-Vazquez; A Ruiz-Carillo
Journal:  Nucleic Acids Res       Date:  1982-03-25       Impact factor: 16.971

Review 6.  Sequence organization of animal nuclear DNA.

Authors:  J Schmidtke; J T Epplen
Journal:  Hum Genet       Date:  1980       Impact factor: 4.132

7.  Multiple forms of H4 histone mRNA in human cells.

Authors:  A C Lichtler; S Detke; I R Phillips; G S Stein; J L Stein
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

8.  Leader sequences of Strongylocentrotus purpuratus histone mRNAs start at a unique heptanucleotide common to all five histone genes.

Authors:  I Sures; S Levy; L H Kedes
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

9.  Isolation and characterization of human actin genes.

Authors:  J N Engel; P W Gunning; L Kedes
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

10.  Human cytoplasmic actin proteins are encoded by a multigene family.

Authors:  J Engel; P Gunning; L Kedes
Journal:  Mol Cell Biol       Date:  1982-06       Impact factor: 4.272
  10 in total

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