Literature DB >> 7954902

A versatile shuttle cosmid vector for the efficient construction of genomic libraries and for the cloning of fungal genes.

H D Osiewacz1.   

Abstract

A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of genomic DNA. Due to the presence of a functional hygromycin B phosphotransferase gene (hph) transformation of fungal protoplasts with pAN-sCos1, or derivatives of it, results in the formation of hygromycin B-resistant transformants. The T7 and T3 RNA polymerase promoter sequences flanking the cloning site, in combination with two adjacent NotI sites facilitate genomic walking and the rapid construction of restriction maps of cloned inserts.

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Year:  1994        PMID： 7954902     DOI： 10.1007/BF00326309

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  7 in total

1.  Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes.

Authors:  H D Osiewacz; A Skaletz; K Esser
Journal:  Appl Microbiol Biotechnol       Date:  1991-04       Impact factor: 4.813

2.  Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors:  P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Genome organization and characterization of the repetitive and inverted repeat DNA sequences in Neurospora crassa.

Authors:  R Krumlauf; G A Marzluf
Journal:  J Biol Chem       Date:  1980-02-10       Impact factor: 5.157

4.  Sequence analysis of the gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of Podospora anserina: use of homologous regulatory sequences to improve transformation efficiency.

Authors:  R Ridder; H D Osiewacz
Journal:  Curr Genet       Date:  1992-03       Impact factor: 3.886

5.  Efficient cloning of genes of Neurospora crassa.

Authors:  S J Vollmer; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

6.  A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus.

Authors:  M M Yelton; W E Timberlake; C A Hondel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

7.  Molecular cloning of the rDNA of Saccharomyces rosei and comparison of its transcription initiation region with that of Saccharomyces carlsbergensis.

Authors:  M P Verbeet; J Klootwijk; H van Heerikhuizen; R Fontijn; E Vreugdenhil; R J Planta
Journal:  Gene       Date:  1983-07       Impact factor: 3.688
  7 in total
  21 in total

1.  BimD/SPO76 is at the interface of cell cycle progression, chromosome morphogenesis, and recombination.

Authors:  D van Heemst; E Kafer; T John; C Heyting; M van Aalderen; D Zickler
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

2.  Versatile EGFP reporter plasmids for cellular localization of recombinant gene products in filamentous fungi.

Authors:  Stefanie Pöggeler; Sandra Masloff; Birgit Hoff; Severine Mayrhofer; Ulrich Kück
Journal:  Curr Genet       Date:  2003-01-31       Impact factor: 3.886

3.  A WD40 repeat protein regulates fungal cell differentiation and can be replaced functionally by the mammalian homologue striatin.

Authors:  Stefanie Pöggeler; Ulrich Kück
Journal:  Eukaryot Cell       Date:  2004-02

4.  A mutant defective in sexual development produces aseptate ascogonia.

Authors:  Sandra Bloemendal; Kathryn M Lord; Christine Rech; Birgit Hoff; Ines Engh; Nick D Read; Ulrich Kück
Journal:  Eukaryot Cell       Date:  2010-10-15

5.  Overexpression of Pa_1_10620 encoding a mitochondrial Podospora anserina protein with homology to superoxide dismutases and ribosomal proteins leads to lifespan extension.

Authors:  Carolin Grimm; Lena Böhl; Heinz D Osiewacz
Journal:  Curr Genet       Date:  2014-08-24       Impact factor: 3.886

6.  GRISEA, a putative copper-activated transcription factor from Podospora anserina involved in differentiation and senescence.

Authors:  H D Osiewacz; U Nuber
Journal:  Mol Gen Genet       Date:  1996-08-27

7.  Differential expression of the Aspergillus fumigatus pksP gene detected in vitro and in vivo with green fluorescent protein.

Authors:  K Langfelder; B Philippe; B Jahn; J P Latgé; A A Brakhage
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

8.  Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development.

Authors:  Andrea Weil; Karin Luce; Stefan Dröse; Ilka Wittig; Ulrich Brandt; Heinz D Osiewacz
Journal:  Cell Cycle       Date:  2011-12-15       Impact factor: 4.534

9.  Cloning and characterization of the eapB and eapC genes of Cryphonectria parasitica encoding two new acid proteinases, and disruption of eapC.

Authors:  P Jara; S Gilbert; P Delmas; J C Guillemot; M Kaghad; P Ferrara; G Loison
Journal:  Mol Gen Genet       Date:  1996-01-15

10.  Cell differentiation during sexual development of the fungus Sordaria macrospora requires ATP citrate lyase activity.

Authors:  M Nowrousian; S Masloff; S Pöggeler; U Kück
Journal:  Mol Cell Biol       Date:  1999-01       Impact factor: 4.272
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