Literature DB >> 795426

Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.

Z Khalkhali, R D Marshall, F Reuvers, C Habets-Willems, P Boer.   

Abstract

Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34--36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.

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Year:  1976        PMID： 795426      PMCID： PMC1164198          DOI： 10.1042/bj1600037

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  BIOSYNTHESIS OF GLYCOPROTEINS. IV. THE SUBCELLULAR SITES OF INCORPORATION OF GLUCOSAMINE-1-14-C INTO GLYCOPROTEIN RAT LIVER.

Authors:  J MOLNAR; G B ROBINSON; R J WINZLER
Journal:  J Biol Chem       Date:  1965-05       Impact factor: 5.157

2.  Chromatography of ribonuclease on carboxymethyl cellulose columns.

Authors:  G TABORSKY
Journal:  J Biol Chem       Date:  1959-10       Impact factor: 5.157

3.  The effect of guanosine diphosphate and triphosphate on the incorporation of labeled amino acids into proteins.

Authors:  E B KELLER; P C ZAMECNIK
Journal:  J Biol Chem       Date:  1956-07       Impact factor: 5.157

4.  Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.

Authors:  J S Tkacz; O Lampen
Journal:  Biochem Biophys Res Commun       Date:  1975-07-08       Impact factor: 3.575

5.  Structure and function of ribonuclease.

Authors:  H A SCHERAGA; J A RUPLEY
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1962

6.  [Purification of trypsinogen and bovine trypsin by chromatography on carboxymethyl cellulose].

Authors:  S MAROUX; M ROVERY; P DESNUELLE
Journal:  Biochim Biophys Acta       Date:  1962-01-01

7.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

8.  Biosynthesis of acid phosphatase of baker's yeast. Factors influencing its production by protoplasts and characterization of the secreted enzyme.

Authors:  H J Van Rijn; P Boer; E P Steyn-Parvé
Journal:  Biochim Biophys Acta       Date:  1972-05-12

Review 9.  The nature and metabolism of the carbohydrate-peptide linkages of glycoproteins.

Authors:  R D Marshall
Journal:  Biochem Soc Symp       Date:  1974

10.  UDP-N-acetyl-D-glucosamine-asparagine sequon N-acetyl-beta-D-glucosaminyl-transferase-activity in human serum.

Authors:  Z Khalkhali; R D Marshall
Journal:  Carbohydr Res       Date:  1976-07       Impact factor: 2.104
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  2 in total

1.  The UDP-N-acetylglucosamine-asparagine-sequon N-acetyl-beta-D-glucosaminyltransferase activity in preparations of rough endoplasmic reticulum from regenerating rat liver.

Authors:  Z Khalkhali; F Serafini-Cessi; R D Marshall
Journal:  Biochem J       Date:  1977-04-15       Impact factor: 3.857

2.  Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity.

Authors:  Tanis Hogg; Jameson T Mendel; Jonathan L Lavezo
Journal:  Mol Med Rep       Date:  2015-07-29       Impact factor: 2.952
  2 in total

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