| Literature DB >> 7952073 |
A J Harker1, G L Evans, A E Hawley, D M Morris.
Abstract
A high-performance liquid chromatographic method for the determination of 2'-deoxy-3'-thiacytidine (3TC), a novel dideoxy-nucleoside analogue, in human serum is described. 3TC was extracted from serum samples using Bond Elut Certify solid-phase extraction cartridges prior to reversed-phase chromatography with UV detection. The method has been shown to be valid over the concentration range 10-5000 ng/ml using a 1-ml sample volume, both before and after heat treatment of the samples at 60 degrees C for 3 h. The method has been automated using a Zymark robot and used in the analysis of serum samples from HIV positive patients.Entities:
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Year: 1994 PMID： 7952073 DOI： 10.1016/0378-4347(94)80092-8
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495