Time course of formation of inositol phosphates during enzymatic hydrolysis of phytic acid (myo-inositol hexaphosphoric acid) by phytase determined by capillary isotachophoresis.

Capillary isotachophoresis with conductivity detection was applied to the investigation of the hydrolytic decomposition of phytic acid (myo-inositol hexaphosphoric acid) by phytase, and for the formation of the reaction products as a function of time. The quantitation of all analytes (besides phytic acid the mono- to penta-phosphorylated inositols and orthophosphate) can be carried out using two different buffer systems.