Literature DB >> 7949339

Optimization by mass spectrometry of a tryptophan-specific protein cleavage reaction.

M M Vestling1, M A Kelly, C Fenselau.   

Abstract

Mass spectrometry has been used to define the reaction of BNPS-skatole (3-bromo-3-methyl-2-(o-nitrophenylsulfenyl)indolenine) with selected proteins and peptides, confirming the cleavage of the protein chain at tryptophan residues, characterizing the products, and identifying the side reactions. A short procedure for carrying out the cleavage reaction with minimal side reactions is presented.

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Year:  1994        PMID： 7949339     DOI： 10.1002/rcm.1290080925

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  4 in total

1.  Tryptophan residue is essential for immunoreactivity of a diagnostically relevant peptide epitope of A. fumigatus.

Authors:  Neel Kamal; Shantanu Chowdhury; Taruna Madan; Deepak Sharma; M Attreyi; Wahajul Haq; Seturam Bandacharya Katti; Anil Kumar; P Usha Sarma
Journal:  Mol Cell Biochem       Date:  2005-07       Impact factor: 3.396

2.  Derivatization of protonated peptides via gas phase ion-molecule reactions with acetone.

Authors:  R A O'Hair; G E Reid
Journal:  J Am Soc Mass Spectrom       Date:  2000-03       Impact factor: 3.262

3.  A large-scale allosteric transition in cytochrome P450 3A4 revealed by luminescence resonance energy transfer (LRET).

Authors:  Elena V Sineva; Jessica A O Rumfeldt; James R Halpert; Dmitri R Davydov
Journal:  PLoS One       Date:  2013-12-23       Impact factor: 3.240

4.  In Silico Proteome Cleavage Reveals Iterative Digestion Strategy for High Sequence Coverage.

Authors:  Jesse G Meyer
Journal:  ISRN Comput Biol       Date:  2014-04-22
  4 in total

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