Literature DB >> 7949213

Demonstration of gene-amplification by PCR in archival paraffin-embedded breast cancer tissue.

U Lönn1, S Lönn, B Nilsson, C Silfverswärd, B Stenkvist.   

Abstract

The polymerase chain reaction (PCR) is a powerful tool to examine genetic alterations in tumor samples. We describe a simple, rapid, nonisotopic PCR method to semi-quantitatively determine the number of gene copies in human formalin-fixed paraffin-embedded tissue. The procedure is exemplified by analysis of 15 years old breast cancer samples to determine the presence of amplification of c-erb-B2. The samples were obtained from routine specimens kept in pathological archives. Patients with amplified samples showed a poor prognosis, both for recurrences and death in breast cancer.

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Year:  1994        PMID： 7949213     DOI： 10.1007/bf00666058

Source DB:  PubMed          Journal:  Breast Cancer Res Treat        ISSN： 0167-6806            Impact factor:   4.872


  22 in total

1.  Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction.

Authors:  D P Jackson; F A Lewis; G R Taylor; A W Boylston; P Quirke
Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

2.  PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time.

Authors:  C E Greer; S L Peterson; N B Kiviat; M M Manos
Journal:  Am J Clin Pathol       Date:  1991-02       Impact factor: 2.493

3.  Human HER2 (neu) promoter: evidence for multiple mechanisms for transcriptional initiation.

Authors:  M Tal; C R King; M H Kraus; A Ullrich; J Schlessinger; D Givol
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272

4.  Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin.

Authors:  T A Libermann; H R Nusbaum; N Razon; R Kris; I Lax; H Soreq; N Whittle; M D Waterfield; A Ullrich; J Schlessinger
Journal:  Nature       Date:  1985 Jan 10-18       Impact factor: 49.962

5.  Correlation between long-term survival in breast cancer patients and amplification of two putative oncogene-coamplification units: hst-1/int-2 and c-erbB-2/ear-1.

Authors:  H Tsuda; S Hirohashi; Y Shimosato; T Hirota; S Tsugane; H Yamamoto; N Miyajima; K Toyoshima; T Yamamoto; J Yokota
Journal:  Cancer Res       Date:  1989-06-01       Impact factor: 12.701

6.  Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene.

Authors:  D J Slamon; G M Clark; S G Wong; W J Levin; A Ullrich; W L McGuire
Journal:  Science       Date:  1987-01-09       Impact factor: 47.728

7.  Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage.

Authors:  G M Brodeur; R C Seeger; M Schwab; H E Varmus; J M Bishop
Journal:  Science       Date:  1984-06-08       Impact factor: 47.728

8.  Rapid detection of MYCN gene amplification in neuroblastomas using the polymerase chain reaction.

Authors:  D C Crabbe; J Peters; R C Seeger
Journal:  Diagn Mol Pathol       Date:  1992-12

9.  L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer.

Authors:  M M Nau; B J Brooks; J Battey; E Sausville; A F Gazdar; I R Kirsch; O W McBride; V Bertness; G F Hollis; J D Minna
Journal:  Nature       Date:  1985 Nov 7-13       Impact factor: 49.962

10.  Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.

Authors:  K E Noonan; C Beck; T A Holzmayer; J E Chin; J S Wunder; I L Andrulis; A F Gazdar; C L Willman; B Griffith; D D Von Hoff; I B Roninson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205
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  1 in total

1.  A differential PCR assay for the detection of c-erbB 2 amplification used in a prospective study of breast cancer.

Authors:  B A Jennings; J E Hadfield; S D Worsley; A Girling; G Willis
Journal:  Mol Pathol       Date:  1997-10
  1 in total

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