Literature DB >> 7948197

Cloning, expression, and sequence analysis of the ORF4 gene of the porcine reproductive and respiratory syndrome virus MN-1b.

J Kwang1, H S Kim, H S Joo.   

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.

Entities:  

Mesh：

Year:  1994        PMID： 7948197     DOI： 10.1177/104063879400600302

Source DB:  PubMed          Journal:  J Vet Diagn Invest        ISSN： 1040-6387            Impact factor:   1.279


  7 in total

1.  Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay.

Authors:  S A Gilbert; R Larochelle; R Magar; H J Cho; D Deregt
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

2.  Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea.

Authors:  Chulseung Lee; Hyekwon Kim; Bokyu Kang; Minjoo Yeom; Sangyoon Han; Hyoungjoon Moon; Seongjun Park; Hyunil Kim; Daesub Song; Bongkyun Park
Journal:  Virus Genes       Date:  2010-04       Impact factor: 2.332

Review 3.  Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development.

Authors:  X J Meng
Journal:  Vet Microbiol       Date:  2000-06-12       Impact factor: 3.293

4.  Molecular characterization of PL97-1, the first Korean isolate of the porcine reproductive and respiratory syndrome virus.

Authors:  Shien-Young Kang; Sang-Im Yun; Hyo-Sun Park; Choi-Kyu Park; Hyo-Seong Choi; Young-Min Lee
Journal:  Virus Res       Date:  2004-09-01       Impact factor: 3.303

Review 5.  Porcine reproductive and respiratory syndrome (PRRS): a review, with emphasis on pathological, virological and diagnostic aspects.

Authors:  S H Done; D J Paton; M E White
Journal:  Br Vet J       Date:  1996-03

6.  Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus, Québec reference strain.

Authors:  H Mardassi; S Mounir; S Dea
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

7.  Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus.

Authors:  I Morozov; X J Meng; P S Paul
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.