Literature DB >> 7945334

Stable expression of the neuronal BI (class A) calcium channel in baby hamster kidney cells.

T Niidome1, T Teramoto, Y Murata, I Tanaka, T Seto, K Sawada, Y Mori, K Katayama.   

Abstract

Plasmids containing the BI (alpha 1) cDNA with the dihydrofolate reductase (DHFR) gene, skeletal muscle alpha 2-subunit cDNA with the neo marker gene, and beta-subunit cDNA were co-transfected into baby hamster kidney (BHK) cells. BHK cells lack endogenous calcium channel activity. Twenty percent of the methotrexate (MTX) and G418 resistant clones were found to express calcium channel activity using the patch-clamp technique. A single clone, BHKBI147, demonstrated stable electrophysiological characteristics over 20 passages. Ca2+ currents of the BI channel in BHKBI147 cells were largely blocked by a specific P-type blocker, omega-AgaIVA, with an IC50 of 150 nM. Unlike the BI channel, Ca2+ currents of cardiac L-type channels expressed in BHK cells were completely blocked by the L-type antagonist, nifedipine, with an IC50 of 56 nM.

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Year:  1994        PMID： 7945334     DOI： 10.1006/bbrc.1994.2399

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Properties of Q-type calcium channels in neostriatal and cortical neurons are correlated with beta subunit expression.

Authors:  P G Mermelstein; R C Foehring; T Tkatch; W J Song; G Baranauskas; D J Surmeier
Journal:  J Neurosci       Date:  1999-09-01       Impact factor: 6.167

2.  Ablation of P/Q-type Ca(2+) channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the alpha(1A)-subunit.

Authors:  K Jun; E S Piedras-Rentería; S M Smith; D B Wheeler; S B Lee; T G Lee; H Chin; M E Adams; R H Scheller; R W Tsien; H S Shin
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

3.  Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.

Authors:  Yoshitsugu Uriu; Shigeki Kiyonaka; Takafumi Miki; Masakuni Yagi; Satoshi Akiyama; Emiko Mori; Akito Nakao; Aaron M Beedle; Kevin P Campbell; Minoru Wakamori; Yasuo Mori
Journal:  J Biol Chem       Date:  2010-05-07       Impact factor: 5.157

4.  FPL 64176 modification of Ca(V)1.2 L-type calcium channels: dissociation of effects on ionic current and gating current.

Authors:  Stefan I McDonough; Yasuo Mori; Bruce P Bean
Journal:  Biophys J       Date:  2004-10-22       Impact factor: 4.033

5.  Two novel alleles of tottering with distinct Ca(v)2.1 calcium channel neuropathologies.

Authors:  T Miki; T A Zwingman; M Wakamori; C M Lutz; S A Cook; D A Hosford; K Herrup; C F Fletcher; Y Mori; W N Frankel; V A Letts
Journal:  Neuroscience       Date:  2008-07-01       Impact factor: 3.590

6.  Effects of calmodulin and Ca2+ channel blockers on omega-conotoxin GVIA binding to crude membranes from alpha1B subunit (Cav2.2) expressed BHK cells and mice brain lacking the alpha1B subunits.

Authors:  Tetsuyuki Wada; Takashi Imanishi; Akinori Kawaguchi; Masayuki X Mori; Yasuo Mori; Keiji Imoto; Seiji Ichida
Journal:  Neurochem Res       Date:  2005-08       Impact factor: 3.996

7.  Reduced voltage sensitivity of activation of P/Q-type Ca2+ channels is associated with the ataxic mouse mutation rolling Nagoya (tg(rol)).

Authors:  Y Mori; M Wakamori; S Oda; C F Fletcher; N Sekiguchi; E Mori; N G Copeland; N A Jenkins; K Matsushita; Z Matsuyama; K Imoto
Journal:  J Neurosci       Date:  2000-08-01       Impact factor: 6.167

8.  Low-affinity blockade of neuronal N-type Ca channels by the spider toxin omega-agatoxin-IVA.

Authors:  S S Sidach; I M Mintz
Journal:  J Neurosci       Date:  2000-10-01       Impact factor: 6.167

9.  BARP suppresses voltage-gated calcium channel activity and Ca2+-evoked exocytosis.

Authors:  Pascal Béguin; Kazuaki Nagashima; Ramasubbu N Mahalakshmi; Réjan Vigot; Atsuko Matsunaga; Takafumi Miki; Mei Yong Ng; Yu Jin Alvin Ng; Chiaw Hwee Lim; Hock Soon Tay; Le-Ann Hwang; Dmitri Firsov; Bor Luen Tang; Nobuya Inagaki; Yasuo Mori; Susumu Seino; Thomas Launey; Walter Hunziker
Journal:  J Cell Biol       Date:  2014-04-21       Impact factor: 10.539
  9 in total

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