Immunofluorimetric and combined immunofluorimetric cytophotometric and autoradiographic investigations on human myeloma cells.

Bone marrow cells from patients with myeloma were reacted with FITC- and/or TRITC-conjugated anti-heavy chain sera and/or TRITC-conjugated anti-light chain sera. A fluorimeter with electronic setter control and short-term excitation was used for quantitative determination of FITC- and TRITC-fluorescence.