Literature DB >> 7940758

The two-hybrid system: an assay for protein-protein interactions.

S Fields1, R Sternglanz.   

Abstract

The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.

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Year:  1994        PMID： 7940758     DOI： 10.1016/0168-9525(90)90012-u

Source DB:  PubMed          Journal:  Trends Genet        ISSN： 0168-9525            Impact factor:   11.639


  189 in total

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7.  Genetic dissection of protein-protein interactions in multi-tRNA synthetase complex.

Authors:  S B Rho; M J Kim; J S Lee; W Seol; H Motegi; S Kim; K Shiba
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-13       Impact factor: 11.205

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