Literature DB >> 7937478

Simple method to purify chicken immunoglobulin G.

J K Bhanushali1, J M Gilbert, L R McDougald.   

Abstract

A new method for purifying IgG was adapted for use with chicken serum. Chicken serum was mixed with caprylic acid, precipitated with ammonium sulfate, and dialyzed against PBS. Analysis by SDS-PAGE and Western blot confirmed successful purification of chicken IgG. Large quantities of highly purified IgG were easily obtainable for use in immunological investigations or for labeling. This technique is rapid, inexpensive, and simpler to perform than traditional ion exchange or gel filtration chromatography.

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Year:  1994        PMID： 7937478     DOI： 10.3382/ps.0731158

Source DB:  PubMed          Journal:  Poult Sci        ISSN： 0032-5791            Impact factor:   3.352


  6 in total

1.  Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils.

Authors:  Kim Thomsen; Lars Christophersen; Peter Østrup Jensen; Thomas Bjarnsholt; Claus Moser; Niels Høiby
Journal:  Hum Vaccin Immunother       Date:  2016-02-22       Impact factor: 3.452

2.  Assessment of cell mediated immune response in rabbits immunized with affinity purified 35 kDa midgut antigen of Rhipicephalus haemaphysaloides ticks.

Authors:  K T Kavitha; B R Latha; G Dhinakar Raj
Journal:  J Parasit Dis       Date:  2013-04-03

3.  Chicken IgY reduces the risk of Pseudomonas aeruginosa urinary tract infections in a murine model.

Authors:  Franziska A Schwartz; Lars Christophersen; Kim Thomsen; Sarah Baekdal; Maria Pals Bendixen; Mette Jørgensen; Ida Kirstine Bull Rasmussen; Anne Sofie Laulund; Niels Høiby; Claus Moser
Journal:  Front Microbiol       Date:  2022-09-09       Impact factor: 6.064

4.  Production of a monoclonal antibody against chicken immunoglobulin G: A valuable molecule with research and diagnostic applications.

Authors:  Masoud Reza Seyfi Abad Shapouri; Mohammad Rashno; Pezhman Mahmoodi; Mahshid Aria
Journal:  Vet Res Forum       Date:  2018-03-15       Impact factor: 1.054

Review 5.  IgY: a promising antibody for use in immunodiagnostic and in immunotherapy.

Authors:  Wilmar Dias da Silva; Denise V Tambourgi
Journal:  Vet Immunol Immunopathol       Date:  2010-01-07       Impact factor: 2.046

Review 6.  Immune Responses to Pseudomonas aeruginosa Biofilm Infections.

Authors:  Claus Moser; Peter Østrup Jensen; Kim Thomsen; Mette Kolpen; Morten Rybtke; Anne Sofie Lauland; Hannah Trøstrup; Tim Tolker-Nielsen
Journal:  Front Immunol       Date:  2021-02-22       Impact factor: 7.561
  6 in total

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