| Literature DB >> 7937114 |
A Jin1, Y Zhang, Y Xia, E Traylor, M Nelson, J L Van Etten.
Abstract
A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.Entities:
Mesh:
Substances:
Year: 1994 PMID: 7937114 PMCID: PMC308391 DOI: 10.1093/nar/22.19.3928
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971