Literature DB >> 7935343

Mutagenesis of the catalytic subunit of rabbit muscle protein phosphatase-1.

Z Zhang1, S Zhao, S Deans-Zirattu, G Bai, E Y Lee.   

Abstract

We have generated site-directed mutants of the catalytic subunit of rabbit muscle ppase-1. Since it is known that ppase-1 and ppase-2A are highly susceptible to inactivation by sulfhydryl reagents, we have mutagenized the six cysteine residues conserved between these two enzymes to serines. The six mutants were purified to near homogeneity by affinity chromatography on inhibitor-2-Sepharose and characterized. All six exhibited enzymatic activity. These results indicate that the catalytic mechanism of ppase-1 is different from that of the protein tyrosine phosphatases which involve a cysteinyl phosphate intermediate.

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Year:  1993        PMID： 7935343     DOI： 10.1007/bf01076762

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  41 in total

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Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

Review 2.  Properties and regulation of liver phosphorylase phosphatase.

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Journal:  Adv Enzyme Regul       Date:  1976

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Authors:  M J Hubbard; P Cohen
Journal:  Eur J Biochem       Date:  1989-12-22

4.  A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.

Authors:  M A Vandeyar; M P Weiner; C J Hutton; C A Batt
Journal:  Gene       Date:  1988-05-15       Impact factor: 3.688

5.  Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A.

Authors:  N Berndt; D G Campbell; F B Caudwell; P Cohen; E F da Cruz e Silva; O B da Cruz e Silva; P T Cohen
Journal:  FEBS Lett       Date:  1987-11-02       Impact factor: 4.124

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Authors:  R L Van Etten
Journal:  Ann N Y Acad Sci       Date:  1982       Impact factor: 5.691

7.  Expression and characterization of rat protein phosphatases-1 alpha, -1 gamma 1, -1 gamma 2, and -1 delta.

Authors:  Z Zhang; G Bai; M Shima; S Zhao; M Nagao; E Y Lee
Journal:  Arch Biochem Biophys       Date:  1993-06       Impact factor: 4.013

Review 8.  The phosphoprotein phosphatases: properties of the enzymes involved in the regulation of glycogen metabolism.

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Journal:  Adv Cyclic Nucleotide Res       Date:  1980

Review 9.  The structure and regulation of protein phosphatases.

Authors:  P Cohen
Journal:  Annu Rev Biochem       Date:  1989       Impact factor: 23.643

10.  Expression of the catalytic subunit of phosphorylase phosphatase (protein phosphatase-1) in Escherichia coli.

Authors:  A J Zhang; G Bai; S Deans-Zirattu; M F Browner; E Y Lee
Journal:  J Biol Chem       Date:  1992-01-25       Impact factor: 5.157
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  7 in total

1.  Isolation and cloning of a protein-serine/threonine phosphatase from an archaeon.

Authors:  J Leng; A J Cameron; S Buckel; P J Kennelly
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

2.  Protein phosphatase 1γ isoforms linked interactions in the brain.

Authors:  Sara L C Esteves; Luís Korrodi-Gregório; Cândida Z Cotrim; Paula J M van Kleeff; Sara C Domingues; Odete A B da Cruz e Silva; Margarida Fardilha; Edgar F da Cruz e Silva
Journal:  J Mol Neurosci       Date:  2012-10-19       Impact factor: 3.444

3.  Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin LR.

Authors:  D G Bourne; G J Jones; R L Blakeley; A Jones; A P Negri; P Riddles
Journal:  Appl Environ Microbiol       Date:  1996-11       Impact factor: 4.792

4.  The protein phosphatases of Synechocystis sp. strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro.

Authors:  Renhui Li; M Ben Potters; Liang Shi; Peter J Kennelly
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

5.  Dictyostelium discoideum protein phosphatase-1 catalytic subunit exhibits distinct biochemical properties.

Authors:  Luiz P M Andrioli; Paulo A Zaini; Wladia Viviani; Aline M Da Silva
Journal:  Biochem J       Date:  2003-08-01       Impact factor: 3.857

Review 6.  Viewing serine/threonine protein phosphatases through the eyes of drug designers.

Authors:  Mengmeng Zhang; S D Yogesha; Joshua E Mayfield; Gordon N Gill; Yan Zhang
Journal:  FEBS J       Date:  2013-09-05       Impact factor: 5.542

Review 7.  Microcystin-LR and cylindrospermopsin induced alterations in chromatin organization of plant cells.

Authors:  Csaba Máthé; Márta M-Hamvas; Gábor Vasas
Journal:  Mar Drugs       Date:  2013-09-30       Impact factor: 5.118
  7 in total

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