Literature DB >> 7930633

Immunization with plasmid DNA using a pneumatic gun.

H L Vahlsing1, M A Yankauckas, M Sawdey, S H Gromkowski, M Manthorpe.   

Abstract

We characterize a method by which the Med-E-Jet pneumatic vaccination gun can be used to propel intact, supercoiled plasmid DNA through skin and into skeletal muscles of mice. Intramuscular injection of plasmids containing the firefly luciferase gene linked to the human cytomegalovirus promoter resulted in the expression of several hundred picograms of luciferase enzyme in quadriceps muscles. Intramuscular injections of a plasmid containing the influenza A nuclear protein gene regulated by the same promoter resulted in the generation of potent and specific anti-nuclear protein humoral and cellular immune responses. This convenient and rapid injection method would be well-suited for genetic immunization of humans.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7930633     DOI： 10.1016/0022-1759(94)90327-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  Enhancing B- and T-cell immune response to a hepatitis C virus E2 DNA vaccine by intramuscular electrical gene transfer.

Authors:  S Zucchelli; S Capone; E Fattori; A Folgori; A Di Marco; D Casimiro; A J Simon; R Laufer; N La Monica; R Cortese; A Nicosia
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

Review 2.  Pharmaceutical approach to somatic gene therapy.

Authors:  F D Ledley
Journal:  Pharm Res       Date:  1996-11       Impact factor: 4.200

3.  Safety and efficacy of DNA vaccines: plasmids vs. minicircles.

Authors:  Sofia Stenler; Pontus Blomberg; C I Edvard Smith
Journal:  Hum Vaccin Immunother       Date:  2014-02-19       Impact factor: 3.452

4.  Synergistic effect of formulated plasmid and needle-free injection for genetic vaccines.

Authors:  K Anwer; K A Earle; M Shi; J Wang; R J Mumper; B Proctor; K Jansa; H C Ledebur; S Davis; W Eaglstein; A P Rolland
Journal:  Pharm Res       Date:  1999-06       Impact factor: 4.200

5.  Low-volume jet injection for efficient nonviral in vivo gene transfer.

Authors:  Wolfgang Walther; Ulrike Stein; Iduna Fichtner; Peter M Schlag
Journal:  Mol Biotechnol       Date:  2004-10       Impact factor: 2.695

6.  Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression.

Authors:  Sofia Stenler; Oscar Pb Wiklander; Maria Badal-Tejedor; Janne Turunen; Joel Z Nordin; David Hallengärd; Britta Wahren; Samir El Andaloussi; Mark W Rutland; C I Edvard Smith; Karin E Lundin; Pontus Blomberg
Journal:  Mol Ther Nucleic Acids       Date:  2014-01-07       Impact factor: 10.183

7.  HER2/neu DNA vaccination by intradermal gene delivery in a mouse tumor model: Gene gun is superior to jet injector in inducing CTL responses and protective immunity.

Authors:  Tam Nguyen-Hoai; Dennis Kobelt; Oliver Hohn; Minh D Vu; Peter M Schlag; Bernd Dörken; Steven Norley; Martin Lipp; Wolfgang Walther; Antonio Pezzutto; Jörg Westermann
Journal:  Oncoimmunology       Date:  2012-12-01       Impact factor: 8.110

8.  Intratumoral low-volume jet-injection for efficient nonviral gene transfer.

Authors:  Wolfgang Walther; Ulrike Stein; Iduna Fichtner; Carsten Voss; Torsten Schmidt; Martin Schleef; Thomas Nellessen; Peter M Schlag
Journal:  Mol Biotechnol       Date:  2002-06       Impact factor: 2.860

9.  A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation.

Authors:  Thomas Eden; Stephan Menzel; Janusz Wesolowski; Philine Bergmann; Marion Nissen; Gudrun Dubberke; Fabienne Seyfried; Birte Albrecht; Friedrich Haag; Friedrich Koch-Nolte
Journal:  Front Immunol       Date:  2018-01-23       Impact factor: 7.561
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.