Literature DB >> 7929450

Isolation of the stable hexameric DnaK.DnaJ complex from Thermus thermophilus.

K Motohashi1, H Taguchi, N Ishii, M Yoshida.   

Abstract

A DnaK homolog (T.DnaK) has been purified as a stable complex with a DnaJ homolog (T.DnaJ) from a thermophilic bacterium, Thermus thermophilus. This complex has an approximate molecular size of 300 kDa and appears to contain three copies of each of T.DnaK and T.DnaJ molecules. Consistently, trigonal ring structures with a diameter (trigonal apex-to-apex) of about 11 nm were observed with electron microscopy. The complex has no endogenously bound AT(D)P and is stable in the presence of Mg-AT(D)P. It possesses a weak ATPase activity and retains about 3 mol of ADP/mole of the complex when incubated with Mg-ATP. This complex is able to interact with the reduced carboxymethylated alpha-lactalbumin which we used as a model unfolded protein.

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Year:  1994        PMID： 7929450

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  12 in total

1.  Heat-inactivated proteins are rescued by the DnaK.J-GrpE set and ClpB chaperones.

Authors:  K Motohashi; Y Watanabe; M Yohda; M Yoshida
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-22       Impact factor: 11.205

2.  Denaturation studies by fluorescence and quenching of thermophilic protein NAD+-glutamate dehydrogenase from Thermus thermophilus HB8.

Authors:  Jose L Ruiz; Juan Ferrer; Carmen Pire; Francisco I Llorca; Maria José Bonete
Journal:  J Protein Chem       Date:  2003-04

Review 3.  Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions.

Authors:  Fritha Hennessy; William S Nicoll; Richard Zimmermann; Michael E Cheetham; Gregory L Blatch
Journal:  Protein Sci       Date:  2005-07       Impact factor: 6.725

4.  Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast Hsp70 proteins.

Authors:  S Kim; B Schilke; E A Craig; A L Horwich
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-27       Impact factor: 11.205

5.  Sequential action of two hsp70 complexes during protein import into mitochondria.

Authors:  M Horst; W Oppliger; S Rospert; H J Schönfeld; G Schatz; A Azem
Journal:  EMBO J       Date:  1997-04-15       Impact factor: 11.598

6.  A lux-based Staphylococcus aureus bioluminescence screening assay for the detection/identification of antibiotics and prediction of antibiotic mechanisms.

Authors:  Zhongjun Yang; Qingyu Cui; Mengge Zhang; Zhiqiang Li; Mingyu Wang; Hai Xu
Journal:  J Antibiot (Tokyo)       Date:  2020-07-16       Impact factor: 2.649

7.  The unique chaperone operon of Thermotoga maritima: cloning and initial characterization of a functional Hsp70 and small heat shock protein.

Authors:  E T Michelini; G C Flynn
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

8.  The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain.

Authors:  Heather Hundley; Helene Eisenman; William Walter; Tara Evans; Yuka Hotokezaka; Martin Wiedmann; Elizabeth Craig
Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-02       Impact factor: 11.205

9.  A DnaK homolog in Myxococcus xanthus is involved in social motility and fruiting body formation.

Authors:  Z Yang; Y Geng; W Shi
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

10.  Identification and characterization of a Hsp70 (DnaK) chaperone system from Meiothermus ruber.

Authors:  M Pleckaityte; E Mistiniene; V Michailoviene; G Zvirblis
Journal:  Mol Genet Genomics       Date:  2003-02-22       Impact factor: 3.291
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