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Literature DB >> 792684

A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.

J D Friesen, J Parker, R J Watson, D Bendiak, S V Reeh, S Pedersen, N P Fiil.

Abstract

A specialized transducing bacteriophage lambdadpolCdap D-9 has been isolated that carries the structural gene for EF-Ts1 (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UVL-inactivated cells has been detected by two-dimensional gel electrophoresis and has been verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. Evidence is presented which suggest that the structural genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit) also lies on lambdadpolCdap D-9.

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Year: 1976 PMID： 792684 DOI： 10.1007/bf00268549

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

17 in total

1. Late steps in the assembly of 30 S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli.

Authors: H Nashimoto; H Uchida
Journal: J Mol Biol Date: 1975-08-15 Impact factor: 5.469

2. Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophages lambdadrifd 18 and lambdadfus-3.

Authors: S Pedersen; S V Reeh
Journal: Mol Gen Genet Date: 1976-03-30

3. Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.

Authors: J D Friesen; J Parker; R J Watson; N P Fiil; S Pedersen
Journal: Mol Gen Genet Date: 1976-02-27

4. Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase.

Authors: J B Kirschbaum; J Scaife
Journal: Mol Gen Genet Date: 1974

5. Temperature-sensitive mutation in regulation of ribonucleic acid synthesis in Escherichia coli.

Authors: M Kuwano; H Endo; M Yamamoto
Journal: J Bacteriol Date: 1972-12 Impact factor: 3.490

6. A mutant of Escherichia coli blocked in peptide elongation: altered elongation factor Ts.

Authors: M Kuwano; H Endo; T Kamiya; K Hori
Journal: J Mol Biol Date: 1974-07-15 Impact factor: 5.469

7. Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli.

Authors: A Böck; D Ruffler; W Piepersberg; H G Wittmann
Journal: Mol Gen Genet Date: 1974

8. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.

Authors: S R Jaskunas; L Lindahl; M Nomura
Journal: Nature Date: 1975-10-09 Impact factor: 49.962

9. New chromosomal location for structural genes of ribosomal proteins.

Authors: R J Watson; J Parker; N P Fiil; J G Flaks; J D Friesen
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

10. Isolation of a specialized transducing bacteriophage lambda carrying the polC locus of Escherichia coli.

Authors: H Shizuya; D M Livingston; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1974-07 Impact factor: 11.205

16 in total

1. Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.

Authors: M Springer; M Graffe; H Hennecke
Journal: Proc Natl Acad Sci U S A Date: 1977-09 Impact factor: 11.205

2. A study of a mutant elongation factor properties of E. coli HAK88 and its mutant elongation factor Tu.

Authors: P G Lemaux; D L Miller
Journal: Mol Gen Genet Date: 1978-02-07

3. Over-synthesis and instability of sigma protein in a merodiploid strain of Escherichia coli.

Authors: R S Hayward; S Fyfe
Journal: Mol Gen Genet Date: 1978-02-07

4. Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.

Authors: G An; D S Bendiak; L A Mamelak; J D Friesen
Journal: Nucleic Acids Res Date: 1981-08-25 Impact factor: 16.971

9. Specialized lambda transducing bacteriophage which carries hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase.

Authors: S E Fishman; K R Kerchief; J Parker
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

10. Non-coordinate expression of the neighbouring genes rplL and rpoB,C of Escherichia coli.

Authors: R S Hayward; S K Fyfe
Journal: Mol Gen Genet Date: 1978-03-20