Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Isolation and characterization of phi80dgal transducing phages that carry gal operator-promoter insertion mutations.

Literature DB >> 792683

Isolation and characterization of phi80dgal transducing phages that carry gal operator-promoter insertion mutations.

Abstract

phi80dgal transducing bacteriophages have been isolated by the F-fusion technique of Press et al. (1971) and gal-operator-promoter insertion mutations have been introduced by homogenate formation. Five different phi80dgal isolates have been studied in more detail. One of the phi80 phages transduces the gal operon and gene aroG as well as at least part of the trp-operon; the gal operon of another phi80dgal transducing phage is inverted with respect to the phi80dgal sequences. Heteroduplex DNA mapping indicates that one of the phi80dgal isolates in addition to the gal operon and a portion of the adjacent chromosomal region carries an IS2-element which is derived from the F'gal episome. The isolated phi80dgal phages may be utilized for preparing pure gal mRNA and insertion-RNA as well as pure gal operon DNA.

Entities: Chemical Disease Gene

Mesh：

Substances：
DNA, Viral
Galactose

Year: 1976 PMID： 792683 DOI： 10.1007/bf00268548

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

22 in total

1. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

Authors: M Fiandt; W Szybalski; M H Malamy
Journal: Mol Gen Genet Date: 1972

2. mRNA distal to polar nonsense and insertion mutation in the gal operon of E. coli.

Authors: P Starlinger; H Saedler; B Rak; E Tillmann; P Venkov; L Waltschewa
Journal: Mol Gen Genet Date: 1973-05-09

3. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?

Authors: F R Blattner; J E Dahlberg
Journal: Nat New Biol Date: 1972-06-21

4. Insertion mutations in the control region of the galactose operon of E. coli. II. Physical characterization of the mutations.

Authors: H J Hirsch; H Saedler; P Starlinger
Journal: Mol Gen Genet Date: 1972

1. Inhibition of bacterial segregation by early functions of phage mu and association of replication protein B with the inner cell membrane.

Authors: C Boeckh; E G Bade; H Delius; J N Reeve
Journal: Mol Gen Genet Date: 1986-03

2. The structure of unstable constitutive revertants of mutant galOP-308::IS2-I.

Authors: H Delius; D Charlier; J Besemer
Journal: Mol Gen Genet Date: 1980

3. Deletions and DNA rearrangements within the transposable DNA element IS2. A model for the creation of palindromic DNA by DNA repair synthesis.

Authors: J Besemer; G Görtz; D Charlier
Journal: Nucleic Acids Res Date: 1980-12-11 Impact factor: 16.971

4. Transcription of insertion elements IS1 and IS2 in vitro.

Authors: J Besemer; C Molzberger
Journal: Mol Gen Genet Date: 1977-05-20

5. Inversion in the lactose region of Escherichia coli K-12.

Authors: D J Savic
Journal: J Bacteriol Date: 1979-11 Impact factor: 3.490

5 in total

Isolation and characterization of phi80dgal transducing phages that carry gal operator-promoter insertion mutations.

1. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.

2. mRNA distal to polar nonsense and insertion mutation in the gal operon of E. coli.

3. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?

4. Insertion mutations in the control region of the galactose operon of E. coli. II. Physical characterization of the mutations.

5. Effect of Rho on transcription of bacterial operons.

Review 6. Current linkage map of Escherichia coli.

7. The enzymes of the galactose operon in Escherichia coli. 3. The size and composition of galactokinase.

8. DNA directed peptide synthesis. V. The cell-free synthesis of a polypeptide with beta-galactosidase activity.

9. [Studies on the glucose effect in the synthesis of the galactose enzyme of Escherichia coli].

10. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.

1. Inhibition of bacterial segregation by early functions of phage mu and association of replication protein B with the inner cell membrane.

2. The structure of unstable constitutive revertants of mutant galOP-308::IS2-I.

3. Deletions and DNA rearrangements within the transposable DNA element IS2. A model for the creation of palindromic DNA by DNA repair synthesis.

4. Transcription of insertion elements IS1 and IS2 in vitro.

5. Inversion in the lactose region of Escherichia coli K-12.