Literature DB >> 7925342

Oligosaccharides derived by keratanase II digestion of bovine articular cartilage keratan sulphates.

G M Brown1, T N Huckerby, I A Nieduszynski.   

Abstract

Alkaline borohydride-reduced keratan sulphate chains from bovine articular cartilage (6-8-year-old animals) were subjected to a limit digest with the enzyme keratanase II. Using 1H-NMR spectroscopy, 25 reduced oligosaccharides deriving from keratan sulphate were shown to have the following structures [GlcNAc(6S)-ol represents N-acetylglucosaminitol 6-O-sulphate]: Gal beta 1-4-GlcNAc(6S)-ol, Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)-ol, Gal(6S)beta 1-4GlcNAc(6S)-ol, Gal-(6S)beta 1-4GlcNAc(6S) beta 1-3Gal beta 1-4GlcNAc(6S)-ol, Gal beta 1-4GlcNAc(6S)beta 1-3Gal(6S)beta 1-4GlcNAc(6S)-ol, Gal(6S)beta 1-4GlcNAc(6S)beta 1-3Gal(6S)1-4GlcNAc(6S)-ol, Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)-ol, Gal beta 1-4-(Fuc alpha 1-3)GlcNAc(6S)beta1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)-ol, Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4(Fuc alpha 1-3)-GlcNAc(6S)-ol, Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)-ol, Gal(6S) beta 1-4GlcNAc-(6S)beta 1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)-ol, Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)beta 1-3Gal(6S)1-4GlcNAc(6S)-ol, Gal beta 1-4GlcNAc(6S)beta 1-6(Gal beta 1-3)GalNAc-ol, Gal beta 1-4GlcNAc(6S) beta1-6(NeuAc2-3Gal beta 1-3)Gal-NAc-ol, Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)beta 1-6(Gal beta 1-3) GalNAc-ol, Gal(6S)beta 1-4GlcNAc-(6S)beta 1-6(Gal beta 1-3)GalNAc-ol, Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)beta 1-6(NeuAc2-3Gal beta 1-3)-GalNAc-ol, Gal(6S)beta 1-4GlcNAc(6S)beta 1-6(NeuAc alpha 2-3Gal beta 1-3)GalNAc-ol, Gal(6S) beta 1-4GlcNAc-(6S)beta 1-3Gal beta 1-4GlcNAc(6S)beta 1-6(Gal beta 1-3)GalNAc- ol, Gal(6S)beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc-(6S)beta 1-6(NeuAc alpha 2-3Gal beta 1-3)GalNAc-ol, NeuAc alpha 2-6Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)-ol, NeuAc alpha 2-3Gal beta 1-4GlcNAc(6S)beta 1-3Gal beta 1-4GlcNAc(6S)-ol, NeuAc alpha 2-6Gal beta 1-4GlcNAc(6S)beta 1-3Gal-(6S)beta 1-4GlcNAc(6S)-ol, NeuAc alpha 2-3Gal beta 1-4GlcNAc(6S)beta 1-3Gal(6S)beta 1-4GlcNAc(6S)-ol and Neu-Ac alpha 2-3Gal(6S)beta 1-4GlcNAc(6S)beta 1-3Gal(6S beta)1-4GlcNAc(6S)-ol. Proton chemical shifts for these oligosaccharides were assigned using one- and two-dimensional NMR spectroscopic methods. These results confirm the findings of Nakazawa et al. [Nakazawa, K., Ito, M., Yamagata, T. and Suzuki, S. (1989) in Keratan sulphate: chemistry, biology and chemical pathology (Greiling, H. and Scott, J.E., eds) pp. 99-110, The Biochemical Society, London], namely that keratanase II cleaves the O-glycosidic bond of a beta(1-3)-linked 6-O-sulphated N-acetylglucosamine.(ABSTRACT TRUNCATED AT 400 WORDS)

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Year:  1994        PMID： 7925342     DOI： 10.1111/j.1432-1033.1994.00281.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  7 in total

1.  Characterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing alpha(2-3)-linked sialic acid and alpha(1-3)-linked fucose. A sulphated variant of the VIM-2 epitope.

Authors:  G M Brown; T N Huckerby; B L Abram; I A Nieduszynski
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

2.  KSGal6ST is essential for the 6-sulfation of galactose within keratan sulfate in early postnatal brain.

Authors:  Hitomi Hoshino; Tahmina Foyez; Shiori Ohtake-Niimi; Yoshiko Takeda-Uchimura; Makoto Michikawa; Kenji Kadomatsu; Kenji Uchimura
Journal:  J Histochem Cytochem       Date:  2013-10-23       Impact factor: 2.479

3.  Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins.

Authors:  E Mori; J L Hedrick; N J Wardrip; T Mori; S Takasaki
Journal:  Glycoconj J       Date:  1998-05       Impact factor: 2.916

4.  Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding.

Authors:  Amanda Weyers; Bo Yang; Kemal Solakyildirim; Vienna Yee; Lingyun Li; Fuming Zhang; Robert J Linhardt
Journal:  FEBS J       Date:  2013-02-28       Impact factor: 5.542

5.  Proteomic and postproteomic characterization of keratan sulfate-glycanated isoforms of thyroglobulin and transferrin uniquely elaborated by papillary thyroid carcinomas.

Authors:  Gaetano Magro; Daniela Perissinotto; Monica Schiappacassi; Steffen Goletz; Albrecht Otto; Eva-Christina Müller; Michele Bisceglia; Gavin Brown; Timothy Ellis; Sebastiano Grasso; Alfonso Colombatti; Roberto Perris
Journal:  Am J Pathol       Date:  2003-07       Impact factor: 4.307

6.  The structure of the keratan sulphate chains attached to fibromodulin isolated from bovine tracheal cartilage: oligosaccharides generated by keratanase II digestion.

Authors:  R M Lauder; T N Huckerby; I A Nieduszynski
Journal:  Glycoconj J       Date:  1995-10       Impact factor: 2.916

Review 7.  Keratan sulfate, a complex glycosaminoglycan with unique functional capability.

Authors:  Bruce Caterson; James Melrose
Journal:  Glycobiology       Date:  2018-04-01       Impact factor: 4.313
  7 in total

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