| Literature DB >> 7920462 |
J Tankovic1, J Duval, P Courvalin.
Abstract
We have constructed a gyrA trans-complementation plasmid, pAT512, by cloning the wild-type gyrA gene of Staphylococcus aureus into the expression vector pAT392. Introduction by electrotransformation of pAT512 into a high-level fluoroquinolone resistant mutant of S. aureus (ciprofloxacin MIC = 16 micrograms ml-1) having a gyrA mutation which results in a Ser-84 to Leu substitution, reduced the MICs of norfloxacin and ciprofloxacin for the host of four- and eight-fold, respectively.Entities:
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Year: 1994 PMID： 7920462 DOI： 10.1111/j.1574-695X.1994.tb00471.x
Source DB: PubMed Journal: FEMS Immunol Med Microbiol ISSN： 0928-8244