Literature DB >> 7918046

Distinguishing beta 2-glycoprotein I dependent (systemic lupus erythematosus type) and independent (syphilis type) anticardiolipin antibody with Tween 20.

J Matsuda1, N Saitoh, K Gohchi, M Gotoh, M Tsukamoto.   

Abstract

We investigated whether or not the use of Tween 20 could help to distinguish beta 2-glycoprotein I (GPI) independent anticardiolipin antibody (aCL) (syphilis-type aCL) from GPI-dependent aCL (SLE-type aCL) in a GPI-dependent/independent aCL ELISA. aCL was positive in all 16 SLE patients and all 15 syphilis patients, who were positive for aCL in the standard ELISA, in the GPI-independent ELISA with Tween 20. GPI-dependent aCL was detected in 12/16 SLE patients by the GPI-dependent ELISA with Tween 20. aCL was not detected in any of the syphilis patients by GPI-dependent ELISAs. On the basis of these results, we recommend that Tween 20 should be used in ELISAs to distinguish GPI-dependent aCL from GPI-independent aCL.

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Year:  1993        PMID： 7918046     DOI： 10.1111/j.1365-2141.1993.tb03226.x

Source DB:  PubMed          Journal:  Br J Haematol        ISSN： 0007-1048            Impact factor:   6.998


  5 in total

Review 1.  Testing for and clinical significance of anticardiolipin antibodies.

Authors:  S W Reddel; S A Krilis
Journal:  Clin Diagn Lab Immunol       Date:  1999-11

2.  Cofactor dependence and isotype distribution of anticardiolipin antibodies in viral infections.

Authors:  H Guglielmone; S Vitozzi; O Elbarcha; E Fernandez
Journal:  Ann Rheum Dis       Date:  2001-05       Impact factor: 19.103

3.  Antiphosphatidylserine antibodies in patients with autoimmune diseases and HIV-infected patients: effects of Tween 20 and relationship with antibodies to beta2-glycoprotein I.

Authors:  C González; A Lestón; B García-Berrocal; A Sánchez-Rodriguez; J A Martín-Oterino; I Alberca; M Cordero; R Jorge; J A Navajo; J M González-Buitrago
Journal:  J Clin Lab Anal       Date:  1999       Impact factor: 2.352

4.  Prevalence of beta 2-glycoprotein I antibody in systemic lupus erythematosus patients with beta 2-glycoprotein I dependent antiphospholipid antibodies.

Authors:  J Matsuda; N Saitoh; M Gotoh; K Gohchi; M Tsukamoto
Journal:  Ann Rheum Dis       Date:  1995-01       Impact factor: 19.103

Review 5.  Antiphospholipid antibodies: paradigm in transition.

Authors:  Lawrence L Horstman; Wenche Jy; Carlos J Bidot; Yeon S Ahn; Roger E Kelley; Robert Zivadinov; Amir H Maghzi; Masoud Etemadifar; Seyed Ali Mousavi; Alireza Minagar
Journal:  J Neuroinflammation       Date:  2009-01-20       Impact factor: 8.322
  5 in total

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