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Literature DB >> 7917498

A consensus Alu repeat probe for physical mapping.

M A Batzer¹, M Alegria-Hartman, P L Deininger.

Abstract

Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes.

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Year: 1994 PMID： 7917498 DOI： 10.1016/1050-3862(94)90058-2

Source DB: PubMed Journal: Genet Anal Tech Appl ISSN： 1050-3862

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Cited

12 in total

1. A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes.

Authors: S Y Wang; M Cruts; J Del-Favero; Y Zhang; F Tissir; M C Potier; D Patterson; D Nizetic; A Bosch; H Chen; L Bennett; X Estivill; A Kessling; S E Antonarakis; C van Broeckhoven
Journal: Genome Res Date: 1999-11 Impact factor: 9.043

2. Cis-acting influences on Alu RNA levels.

Authors: C Alemán; A M Roy-Engel; T H Shaikh; P L Deininger
Journal: Nucleic Acids Res Date: 2000-12-01 Impact factor: 16.971

3. Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning.

Authors: Sun-Hee Leem; Vladimir N Noskov; Jung-Eun Park; Seung Il Kim; Vladimir Larionov; Natalay Kouprina
Journal: Nucleic Acids Res Date: 2003-03-15 Impact factor: 16.971

4. The decline in human Alu retroposition was accompanied by an asymmetric decrease in SRP9/14 binding to dimeric Alu RNA and increased expression of small cytoplasmic Alu RNA.

Authors: J Sarrowa; D Y Chang; R J Maraia
Journal: Mol Cell Biol Date: 1997-03 Impact factor: 4.272

5. Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning.

Authors: V Larionov; N Kouprina; J Graves; M A Resnick
Journal: Proc Natl Acad Sci U S A Date: 1996-11-26 Impact factor: 11.205

A consensus Alu repeat probe for physical mapping.

1. A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes.

2. Cis-acting influences on Alu RNA levels.

3. Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning.

4. The decline in human Alu retroposition was accompanied by an asymmetric decrease in SRP9/14 binding to dimeric Alu RNA and increased expression of small cytoplasmic Alu RNA.

5. Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning.

6. Small tumor virus genomes are integrated near nuclear matrix attachment regions in transformed cells.

7. The role and amplification of the HS Alu subfamily founder gene.

8. Inverted Alu repeats unstable in yeast are excluded from the human genome.

9. A novel role in DNA metabolism for the binding of Fen1/Rad27 to PCNA and implications for genetic risk.

10. A novel strategy for analysis of gene homologues and segmental genome duplications.