Literature DB >> 791666

Enrichment in spontaneous and complement dependent rosette forming cell populations using Ficoll-Hypaque gradient centrifugation.

E D Carosella, I Paulone, R W Rohwedder, A E Bachmann.   

Abstract

Peripheral blood human T (TL) and B (BL) lymphocytes were separated by continuous Ficoll-Hypaque (FH) gradients. BL were found at the 1050 density interfase (70.5% EAC rosettes) with no TL (0% EAC rosettes); while at the 1068 density interfase there was an enrichment of TL (68% E rosettes) with very few BL (8.5% EAC rosettes).

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Year:  1976        PMID： 791666     DOI： 10.1007/bf01937450

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  11 in total

1.  A rapid method for the isolation of functional thymus-derived murine lymphocytes.

Authors:  M H Julius; E Simpson; L A Herzenberg
Journal:  Eur J Immunol       Date:  1973-10       Impact factor: 5.532

2.  Isolation of subpopulations of lymphocytic cells by the use of isotonically balanced solutions of Ficoll. I. Development of methods and demonstration of the existence of a large but finite number of subpopulations.

Authors:  M K Bach; J R Brashler
Journal:  Exp Cell Res       Date:  1970-08       Impact factor: 3.905

3.  The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation.

Authors:  K Shortman
Journal:  Aust J Exp Biol Med Sci       Date:  1968-08

4.  Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll.

Authors:  R M Gorczynski; R G Miller; R A Phillips
Journal:  Immunology       Date:  1970-11       Impact factor: 7.397

5.  Density distribution analysis of cells forming 19S hemolytic antibody in the rat.

Authors:  J S Haskill; D G Legge; K Shortman
Journal:  J Immunol       Date:  1969-03       Impact factor: 5.422

6.  The separation of different cell classes from lymphoid organs. VI. The effect of osmolarity of gradient media on the density distribution of cells.

Authors:  N Williams; N Kraft; K Shortman
Journal:  Immunology       Date:  1972-05       Impact factor: 7.397

7.  Identification and characterization of subpopulations of lymphocytes in human peripheral blood after fractionation on discontinuous gradients of albumin. The cellular defect in X-linked agammaglobulinemia.

Authors:  R S Geha; F S Rosen; E Merler
Journal:  J Clin Invest       Date:  1973-07       Impact factor: 14.808

8.  A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization.

Authors:  C Bianco; R Patrick; V Nussenzweig
Journal:  J Exp Med       Date:  1970-10-01       Impact factor: 14.307

9.  Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.

Authors:  M Jondal; G Holm; H Wigzell
Journal:  J Exp Med       Date:  1972-08-01       Impact factor: 14.307

10.  Identification of a novel cell type in peripheral lymphoid organs of mice. II. Functional properties in vitro.

Authors:  R M Steinman; Z A Cohn
Journal:  J Exp Med       Date:  1974-02-01       Impact factor: 14.307
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