OBJECTIVE: To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid. METHODS: We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences. RESULTS: Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region. CONCLUSION: The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.
OBJECTIVE: To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid. METHODS: We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences. RESULTS: Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region. CONCLUSION: The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.
Authors: V Krenn; A König; F Hensel; C Berek; M M Souto Carneiro; W Haedicke; Y Wang; H Vollmers; H K Müller-Hermelink Journal: Clin Exp Immunol Date: 1999-01 Impact factor: 4.330
Authors: W J E Van Esch; C C Reparon-Schuijt; H J Hamstra; C Van Kooten; T Logtenberg; F C Breedveld; C L Verweij Journal: Clin Exp Immunol Date: 2003-02 Impact factor: 4.330