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Literature DB >> 791545

A simple immunoenzymatic method for measuring IgE in human sera.

Abstract

A reliable and relatively simple method for the estimation of serum IgE by single radial immunodiffusion is described. The method requires glucose oxidase-labelled antibodies. The method permits the measurement of IgE concentrations ranging from 20 to 700 i.u./ml. The values obtained in unknown samples were in good agreement with those obtained by radioimmunoassay (correlation coefficient r=0-9557).

Entities: Gene Species

Mesh：

Substances：

Year: 1976 PMID： 791545 PMCID： PMC1541397

Source DB: PubMed Journal: Clin Exp Immunol ISSN： 0009-9104 Impact factor: 4.330

11 in total

1. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.

Authors: J L FAHEY; E M MCKELVEY
Journal: J Immunol Date: 1965-01 Impact factor: 5.422

2. Properties of myeloma immunoglobulin E(Yu). Chemical, fluorescence polarisation and spin-labeled studies.

Authors: R S Nezlin; Y A Zagyansky; A I Käiväräinen; D V Stefani
Journal: Immunochemistry Date: 1973-10

3. Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde.

Authors: T Ternynck; S Avrameas
Journal: FEBS Lett Date: 1972-06-01 Impact factor: 4.124

4. A simplified method for measuring human IgE.

Authors: Y M Centifanto; H E Kaufman
Journal: J Immunol Date: 1971-08 Impact factor: 5.422

5. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

Authors: S Avrameas; T Ternynck
Journal: Immunochemistry Date: 1969-01

6. Radioactive single radial diffusion: a method for increasing the sensitivity of immunochemical quantification of proteins in agar gel.

Authors: D S Rowe
Journal: Bull World Health Organ Date: 1969-04 Impact factor: 9.408

7. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors: S Avrameas
Journal: Immunochemistry Date: 1969-01

A simple immunoenzymatic method for measuring IgE in human sera.

1. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.

2. Properties of myeloma immunoglobulin E(Yu). Chemical, fluorescence polarisation and spin-labeled studies.

3. Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde.

4. A simplified method for measuring human IgE.

5. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.

6. Radioactive single radial diffusion: a method for increasing the sensitivity of immunochemical quantification of proteins in agar gel.

7. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

8. An immunoenzymatic method for measuring low concentrations of antigens by single radial diffusion.

9. Measurement of serum IgE by a one-step single radial radiodiffusion method.

10. Studies on single radial immunodiffusion techniques for the quantitation of antigen and antibody.

1. Enzyme-linked immunosorbent assay for measuring serum IgE.