| Literature DB >> 7914093 |
N Monzini1, G Legname, F Marcucci, G Gromo, D Modena.
Abstract
We have identified a heat-shock-inducible 10 kDa protein in the human hepatoma cell line HepG2. The total RNA extracted from the heat-shocked cells was amplified by reverse transcription PCR (polymerase chain reaction) using 21 5' and 18 3' oligonucleotides of rat cpn10 (chaperonin10) cDNA as primers. Sequencing of the above PCR fragment showed a very high homology between human, bovine and rat cpn10 cDNA. The predicted amino acid sequence revealed a 100% identity with the bovine homologue.Entities:
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Year: 1994 PMID： 7914093 DOI： 10.1016/0167-4781(94)90211-9
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002