Literature DB >> 791366

Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.

E H Lo, M H Ng, W S Ng, S Lui, H C Ho.   

Abstract

Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.

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Year:  1976        PMID： 791366     DOI： 10.1016/0304-4165(76)90332-9

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

1.  Antigenic content of an in vitro cellular immunity reactive cell surface extract of Raji cells.

Authors:  M H Ng; W S Ng; H C Ho
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574

2.  A study of Epstein Barr virus receptor activity in cell free extracts of human lymphoblastoid cells.

Authors:  M H Ng; S W Lui; K H Chan
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574

3.  Glycoproteins and sialyl transferase of human B lymphoblastoid cell lines.

Authors:  S W Lui; M H Ng
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574

4.  In vitro immune responses to PPD, extracts from Raji cells and nasopharyngeal carcinoma biopsies in NPC leucocytes.

Authors:  W S Ng; M H Ng; H C Ho; J P Lamelin
Journal:  Br J Cancer       Date:  1977-12       Impact factor: 7.640
  4 in total

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