Literature DB >> 7910736

A rapid and simple method for the purification of transglutaminase from Streptoverticillium mobaraense.

U Gerber1, U Jucknischke, S Putzien, H L Fuchsbauer.   

Abstract

Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.

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Year:  1994        PMID: 7910736      PMCID: PMC1138095          DOI: 10.1042/bj2990825

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  14 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Amino acid sequence of guinea pig liver transglutaminase from its cDNA sequence.

Authors:  K Ikura; T Nasu; H Yokota; Y Tsuchiya; R Sasaki; H Chiba
Journal:  Biochemistry       Date:  1988-04-19       Impact factor: 3.162

3.  [Nepsilon-(beta-Aspartyl)lysine and Nepsilon-(gamma-glutamyl)lysine crosslinkings in bovine colostrum (author's transl)].

Authors:  H Klostermeyer; K Rabbel; E H Reimerdes
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1976-08

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 5.  Transglutaminases.

Authors:  L Lorand; S M Conrad
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

Review 6.  Transglutaminases.

Authors:  J E Folk
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

7.  Transglutaminases.

Authors:  J E Folk; S I Chung
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

Review 8.  The epsilon-(gamma-glutamyl)lysine crosslink and the catalytic role of transglutaminases.

Authors:  J E Folk; J S Finlayson
Journal:  Adv Protein Chem       Date:  1977

Review 9.  Molecular and catalytic properties of transglutaminases.

Authors:  J E Folk; S I Chung
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1973

10.  Nepsilon-(beta-Aspartyl)lysinuria in children with various pathological conditions.

Authors:  J P Kamerling; G Aarsen; P K de Bree; M Duran; S K Wadman; F G Vliegenthart; G I Tesser; K M van der Waarde
Journal:  Clin Chim Acta       Date:  1978-01-02       Impact factor: 3.786

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  12 in total

1.  Characterization and large-scale production of recombinant Streptoverticillium platensis transglutaminase.

Authors:  Shie-Jea Lin; Yi-Fang Hsieh; Li-An Lai; Mei-Li Chao; Wen-Shen Chu
Journal:  J Ind Microbiol Biotechnol       Date:  2008-05-24       Impact factor: 3.346

2.  Determination of mTG Activity in Low-Fat Semi-Hard Cheese Using Fluorescent Labelling.

Authors:  Lívia Darnay
Journal:  J Fluoresc       Date:  2016-12-19       Impact factor: 2.217

3.  Structure of the Dispase Autolysis-inducing Protein from Streptomyces mobaraensis and Glutamine Cross-linking Sites for Transglutaminase.

Authors:  David Fiebig; Stefan Schmelz; Stephan Zindel; Vera Ehret; Jan Beck; Aileen Ebenig; Marina Ehret; Sabrina Fröls; Felicitas Pfeifer; Harald Kolmar; Hans-Lothar Fuchsbauer; Andrea Scrima
Journal:  J Biol Chem       Date:  2016-08-04       Impact factor: 5.157

4.  Illuminating structure and acyl donor sites of a physiological transglutaminase substrate from Streptomyces mobaraensis.

Authors:  Norbert E Juettner; Stefan Schmelz; Jan P Bogen; Dominic Happel; Wolf-Dieter Fessner; Felicitas Pfeifer; Hans-Lothar Fuchsbauer; Andrea Scrima
Journal:  Protein Sci       Date:  2018-03-22       Impact factor: 6.725

Review 5.  Transglutaminases: part I-origins, sources, and biotechnological characteristics.

Authors:  Lovaine Duarte; Carla Roberta Matte; Cristiano Valim Bizarro; Marco Antônio Záchia Ayub
Journal:  World J Microbiol Biotechnol       Date:  2020-01-02       Impact factor: 3.312

6.  Specific mutation of transglutaminase gene from Streptomyces hygroscopicus H197 and characterization of microbial transglutaminase.

Authors:  Wenjie Wan; Donglan He; Zhijun Xue; Zewen Zhang
Journal:  J Biosci       Date:  2017-12       Impact factor: 1.826

7.  Solid state bioreactor production of transglutaminase by Amazonian Bacillus circulans BL32 strain.

Authors:  Claucia Fernanda Volken de Souza; Júlio Xandro Heck; Marco Antônio Záchia Ayub
Journal:  J Ind Microbiol Biotechnol       Date:  2008-08-12       Impact factor: 3.346

8.  Inhibition of α-ketoglutarate-and pyruvate dehydrogenase complexes in E. coli by a glutathione S-transferase containing a pathological length poly-Q domain: A possible role of energy deficit in neurological diseases associated with poly-Q expansions?

Authors:  A J Cooper; K F Sheu; J R Burke; O Onodera; W J Strittmatter; A D Roses; J P Blass
Journal:  Age (Omaha)       Date:  1998-01

Review 9.  Transglutaminases: nature's biological glues.

Authors:  Martin Griffin; Rita Casadio; Carlo M Bergamini
Journal:  Biochem J       Date:  2002-12-01       Impact factor: 3.857

Review 10.  Microbial transglutaminase and its application in the food industry. A review.

Authors:  Marek Kieliszek; Anna Misiewicz
Journal:  Folia Microbiol (Praha)       Date:  2013-11-08       Impact factor: 2.099

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