Alpha 2-adrenoceptors in the guinea-pig uterus: heterogeneity in the circular and longitudinal smooth muscle layers.

Homogenate binding and functional studies have been undertaken to investigate the role of alpha 2-adrenoceptors on the circular and longitudinal myometrial layers of the dioestrous guinea-pig uterus. Each myometrial layer contained a single population of [3H]rauwolscine binding sites (KD values approximately 3 nM) for which yohimbine exhibited a higher affinity than prazosin, and xylazine a higher affinity than phenylephrine, indicating the presence of alpha 2-adrenoceptor binding sites. In circular myometrium, xylazine enhanced contractile responses, was more potent than either noradrenaline or phenylephrine in inhibiting forskolin-stimulated accumulation of cyclic AMP, and reduced the inhibitory effect of forskolin on phenylephrine-induced phosphatidyl inositol hydrolysis. In longitudinal myometrium xylazine enhanced contractile responses to phenylephrine but did not inhibit forskolin-stimulated accumulation of cyclic AMP. We conclude that alpha 2-adrenoceptor binding sites are present in both uterine layers and mediate uterine contractility possibly through different mechanisms.