Poly(ADP-ribose) polymerase gene on chromosome 1q: early role in differentiation linked replication; gene on human chromosome 13q: marker of carcinogenesis.

We have focused in part on utilizing molecular aspects of the PADPRP gene sequences located on chromosome 1q as well as on 13 to help clarify the biological role of these genes. With respect to chromosome 1q, stably transfected cell lines have been established in-both HeLa as well as 3T3-L1 preadipocyte cells, which express a reverse complementary transcription product in vivo to the exons coded for on the chromosome 1 locus. Also, in the case of the PADPRP-like gene on chromosome 13q, we have fine mapped this sequence and have observed in a linkage of deletion of this region with predisposition to various forms of cancer, especially in the African-American population.