| Literature DB >> 789775 |
Abstract
Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.Entities:
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Year: 1976 PMID： 789775 DOI： 10.1016/0022-1759(76)90153-8
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303