Literature DB >> 7892195

Gonadotrophin-releasing hormone receptor agonist-mediated down-regulation of Gq alpha/G11 alpha (pertussis toxin-insensitive) G proteins in alpha T3-1 gonadotroph cells reflects increased G protein turnover but not alterations in mRNA levels.

B H Shah1, D J MacEwan, G Milligan.   

Abstract

Prolonged exposure of alpha T3-1 pituitary gonadotrophs to a gonadotrophin-releasing hormone receptor agonist results in marked down-regulation of the pertussis toxin-insensitive G proteins Gq alpha and G11 alpha. The turnover of Gq alpha/G11 alpha was substantially accelerated in the presence of agonist. By contrast, the rate of degradation of the G protein Gi2 alpha was unaffected by agonist treatment. Analysis of Gq alpha/G11 alpha mRNA levels by reverse transcription-PCR demonstrated no detectable differences between control and agonist-treated cells. These studies indicate that gonadotrophin-releasing hormone receptor agonist-mediated down-regulation of Gq alpha/G11 alpha is a reflection of enhanced proteolysis of the activated G proteins.

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Year:  1995        PMID： 7892195      PMCID： PMC42387          DOI： 10.1073/pnas.92.6.1886

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Cross-regulation between G-protein-mediated pathways. Stimulation of adenylyl cyclase increases expression of the inhibitory G-protein, Gi alpha 2.

Authors:  J R Hadcock; M Ros; D C Watkins; C C Malbon
Journal:  J Biol Chem       Date:  1990-09-05       Impact factor: 5.157

2.  Down-regulation of Gi sub-types by prolonged incubation of adipocytes with an A1 adenosine receptor agonist.

Authors:  A Green; J L Johnson; G Milligan
Journal:  J Biol Chem       Date:  1990-03-25       Impact factor: 5.157

3.  Delta-opioid-receptor-mediated inhibition of adenylate cyclase is transduced specifically by the guanine-nucleotide-binding protein Gi2.

Authors:  F R McKenzie; G Milligan
Journal:  Biochem J       Date:  1990-04-15       Impact factor: 3.857

4.  Cholera toxin treatment produces down-regulation of the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs).

Authors:  G Milligan; C G Unson; M J Wakelam
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

5.  Enhanced degradation of the phosphoinositidase C-linked guanine-nucleotide-binding protein Gq alpha/G11 alpha following activation of the human M1 muscarinic acetylcholine receptor expressed in CHO cells.

Authors:  F M Mitchell; N J Buckley; G Milligan
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

6.  Prostaglandin E1-mediated, cyclic AMP-independent, down-regulation of Gs alpha in neuroblastoma x glioma hybrid cells.

Authors:  F R McKenzie; G Milligan
Journal:  J Biol Chem       Date:  1990-10-05       Impact factor: 5.157

7.  Cholera toxin induces cAMP-independent degradation of Gs.

Authors:  F H Chang; H R Bourne
Journal:  J Biol Chem       Date:  1989-04-05       Impact factor: 5.157

8.  Palmitoylation is required for signaling functions and membrane attachment of Gq alpha and Gs alpha.

Authors:  P B Wedegaertner; D H Chu; P T Wilson; M J Levis; H R Bourne
Journal:  J Biol Chem       Date:  1993-11-25       Impact factor: 5.157

9.  Increased palmitoylation of the Gs protein alpha subunit after activation by the beta-adrenergic receptor or cholera toxin.

Authors:  M Y Degtyarev; A M Spiegel; T L Jones
Journal:  J Biol Chem       Date:  1993-11-15       Impact factor: 5.157

10.  Receptor regulation of G-protein palmitoylation.

Authors:  S M Mumby; C Kleuss; A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-29       Impact factor: 11.205
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  7 in total

1.  Selective down-regulation of the G(q)alpha/G11alpha G-protein family in tumour necrosis factor-alpha induced cell death.

Authors:  V P Pollock; E J Lofthouse; O J Jupp; S B Gauld; H M Anderson; D J MacEwan
Journal:  Mol Cell Biochem       Date:  2000-03       Impact factor: 3.396

2.  Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation.

Authors:  T Drmota; J Novotny; G W Gould; P Svoboda; G Milligan
Journal:  Biochem J       Date:  1999-06-01       Impact factor: 3.857

Review 3.  GnRH signaling, the gonadotrope and endocrine control of fertility.

Authors:  Stuart P Bliss; Amy M Navratil; Jianjun Xie; Mark S Roberson
Journal:  Front Neuroendocrinol       Date:  2010-05-06       Impact factor: 8.606

4.  High-glucose incubation of human umbilical-vein endothelial cells does not alter expression and function either of G-protein alpha-subunits or of endothelial NO synthase.

Authors:  G Mancusi; C Hutter; S Baumgartner-Parzer; K Schmidt; W Schütz; V Sexl
Journal:  Biochem J       Date:  1996-04-01       Impact factor: 3.857

5.  A constitutively active mutant of the alpha 1B-adrenergic receptor can cause greater agonist-dependent down-regulation of the G-proteins G9 alpha and G11 alpha than the wild-type receptor.

Authors:  T W Lee; A Wise; S Cotecchia; G Milligan
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

6.  Signaling responses to pulsatile gonadotropin-releasing hormone in LbetaT2 gonadotrope cells.

Authors:  Rie Tsutsumi; Devendra Mistry; Nicholas J G Webster
Journal:  J Biol Chem       Date:  2010-04-20       Impact factor: 5.157

7.  An agonist-induced switch in G protein coupling of the gonadotropin-releasing hormone receptor regulates pulsatile neuropeptide secretion.

Authors:  Lazar Z Krsmanovic; Nadia Mores; Carlos E Navarro; Krishan K Arora; Kevin J Catt
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-18       Impact factor: 11.205
  7 in total

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