| Literature DB >> 7886450 |
A Dessen1, A Quémard, J S Blanchard, W R Jacobs, J C Sacchettini.
Abstract
Resistance to isoniazid in Mycobacterium tuberculosis can be mediated by substitution of alanine for serine 94 in the InhA protein, the drug's primary target. InhA was shown to catalyze the beta-nicotinamide adenine dinucleotide (NADH)-specific reduction of 2-trans-enoyl-acyl carrier protein, an essential step in fatty acid elongation. Kinetic analyses suggested that isoniazid resistance is due to a decreased affinity of the mutant protein for NADH. The three-dimensional structures of wild-type and mutant InhA, refined to 2.2 and 2.7 angstroms, respectively, revealed that drug resistance is directly related to a perturbation in the hydrogen-bonding network that stabilizes NADH binding.Entities:
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Year: 1995 PMID： 7886450 DOI： 10.1126/science.7886450
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728