Literature DB >> 7883903

Optimization of quantitative culture assay for human immunodeficiency virus from plasma. Plasma Viremia Group Laboratories of the AIDS Clinical Trials Group (National Institute of Allergy and Infectious Diseases).

J L Lathey1, S A Fiscus, S Rasheed, J C Kappes, B P Griffith, T Elbeik, S A Spector, P S Reichelderfer.   

Abstract

Experimental conditions essential to a simple, reproducible, quantitative human immunodeficiency virus plasma assay were determined. Five parameters were evaluated: length of culture, reproducibility, assay dilution schema, washing procedures, and anticoagulant usage. The recommended quantitative plasma assay utilizes undiluted citrated plasma cultured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.

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Year:  1994        PMID： 7883903      PMCID： PMC264230          DOI： 10.1128/jcm.32.12.3064-3067.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

Review 1.  Retroviral proteinases.

Authors:  S Oroszlan; R B Luftig
Journal:  Curr Top Microbiol Immunol       Date:  1990       Impact factor: 4.291

Review 2.  Use of virologic assays for detection of human immunodeficiency virus in clinical trials: recommendations of the AIDS Clinical Trials Group Virology Committee.

Authors:  S Hammer; C Crumpacker; R D'Aquila; B Jackson; J Lathey; D Livnat; P Reichelderfer
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

3.  Quantitation of human immunodeficiency virus type 1 in the blood of infected persons.

Authors:  D D Ho; T Moudgil; M Alam
Journal:  N Engl J Med       Date:  1989-12-14       Impact factor: 91.245

4.  High-level viremia in adults and children infected with human immunodeficiency virus: relation to disease stage and CD4+ lymphocyte levels.

Authors:  M S Saag; M J Crain; W D Decker; S Campbell-Hill; S Robinson; W E Brown; M Leuther; R J Whitley; B H Hahn; G M Shaw
Journal:  J Infect Dis       Date:  1991-07       Impact factor: 5.226

5.  Isolation of HIV-1 from plasma of infected individuals: an analysis of experimental conditions affecting successful virus propagation.

Authors:  R L Dewar; M D Sarmiento; E S Lawton; H M Clark; P E Kennedy; A Shah; M Baseler; J A Metcalf; H C Lane; N P Salzman
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1992

6.  Detection of plasma viremia in human immunodeficiency virus-infected individuals at all clinical stages.

Authors:  L Z Pan; A Werner; J A Levy
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

7.  Effect of didanosine on human immunodeficiency virus viremia and antigenemia in patients with advanced disease: correlation with clinical response.

Authors:  D H Shepp; A Ashraf
Journal:  J Infect Dis       Date:  1993-01       Impact factor: 5.226

8.  Inhibition of human immunodeficiency virus gene amplification by heparin.

Authors:  M Holodniy; S Kim; D Katzenstein; M Konrad; E Groves; T C Merigan
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

9.  Cell-free plasma human immunodeficiency virus type 1 titer assessed by culture and immunocapture-reverse transcription-polymerase chain reaction.

Authors:  R W Coombs; D R Henrard; W F Mehaffey; J Gibson; E Eggert; T C Quinn; J Phillips
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

10.  Loss of infectivity ex vivo in plasma of human immunodeficiency virus-infected patients correlates with a high CD4 cell count.

Authors:  P L Vernazza; J J Eron; S A Fiscus
Journal:  J Infect Dis       Date:  1993-11       Impact factor: 5.226
  10 in total
  3 in total

1.  Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.

Authors:  W A Scott; D Brambilla; E Siwak; C Beatty; J Bremer; R W Coombs; H Farzadegan; S A Fiscus; S M Hammer; F B Hollinger; N Khan; S Rasheed; P S Reichelderfer
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

2.  CD44 microbeads accelerate HIV-1 infection in T cells.

Authors:  Valeri H Terry; Ian C D Johnston; Celsa A Spina
Journal:  Virology       Date:  2009-04-23       Impact factor: 3.616

3.  Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients.

Authors:  Beatrice Macchi; Caterina Frezza; Francesca Marino-Merlo; Antonella Minutolo; Valeria Stefanizzi; Emanuela Balestrieri; Carlotta Cerva; Loredana Sarmati; Massimo Andreoni; Sandro Grelli; Antonio Mastino
Journal:  Pathogens       Date:  2020-12-13
  3 in total

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