Literature DB >> 7882966

The 8.5 A projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits.

S Karrasch1, P A Bullough, R Ghosh.   

Abstract

Two-dimensional crystals from light-harvesting complex I (LHC I) of the purple non-sulfur bacterium Rhodospirillum rubrum have been reconstituted from detergent-solubilized protein complexes. Frozen-hydrated samples have been analysed by electron microscopy. The crystals diffract beyond 8 A and a projection map was calculated to 8.5 A. The projection map shows 16 subunits in a 116 A diameter ring with a 68 A hole in the centre. These dimensions are sufficient to incorporate a reaction centre in vivo. Within each subunit, density for the alpha- and the beta-polypeptide chains is clearly resolved, and the density for the bacteriochlorophylls can be assigned. The experimentally determined structure contradicts models of the LHC I presented so far.

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Year:  1995        PMID： 7882966      PMCID： PMC398126          DOI： 10.1002/j.1460-2075.1995.tb07041.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  23 in total

1.  Purification and crystallization of the light harvesting LH1 complex from Rhodobacter sphaeroides.

Authors:  R S Nunn; P J Artymiuk; P J Baker; D W Rice; C N Hunter
Journal:  J Mol Biol       Date:  1992-12-20       Impact factor: 5.469

2.  Stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris.

Authors:  H Engelhardt; A Engel; W Baumeister
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

3.  Molecular structure determination by electron microscopy of unstained crystalline specimens.

Authors:  P N Unwin; R Henderson
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

4.  High-resolution spot-scan electron microscopy of microcrystals of an alpha-helical coiled-coil protein.

Authors:  P A Bullough; P A Tulloch
Journal:  J Mol Biol       Date:  1990-09-05       Impact factor: 5.469

5.  The light-harvesting polypeptides of Rhodospirillum rubrum. I. The amino-acid sequence of the second light-harvestng polypeptide B 880-beta (B 870-beta) of Rhodospirillum rubrum S 1 and the carotenoidless mutant G-9+. carotenoidless mutant G-9+.

Authors:  R A Brunisholz; F Suter; H Zuber
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1984-07

6.  Isolation and characterization of a bacteriochlorophyll-associated chromatophore protein from Rhodospirillum rubrum G-9.

Authors:  P A Cuendet; H Zuber
Journal:  FEBS Lett       Date:  1977-07-01       Impact factor: 4.124

7.  Projection structure of rhodopsin.

Authors:  G F Schertler; C Villa; R Henderson
Journal:  Nature       Date:  1993-04-22       Impact factor: 49.962

8.  Transfer of chromosomal genes mediated by plasmid r68.45 in Rhodopseudomonas sphaeroides.

Authors:  W R Sistrom
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

9.  Membrane topology of light-harvesting protein B870-alpha of Rhodospirillum rubrum G-9+. Amino acid residues in contact with the lipid bilayer as inferred from labeling with photogenerated carbenes.

Authors:  H Meister; R Bachofen; G Semenza; J Brunner
Journal:  J Biol Chem       Date:  1985-12-25       Impact factor: 5.157

10.  The structure of the photoreceptor unit of Rhodopseudomonas viridis.

Authors:  W Stark; W Kühlbrandt; I Wildhaber; E Wehrli; K Mühlethaler
Journal:  EMBO J       Date:  1984-04       Impact factor: 11.598
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  99 in total

1.  Exciton delocalization in the B808-866 antenna of the green bacterium Chloroflexus aurantiacus as revealed by ultrafast pump-probe spectroscopy.

Authors:  V Novoderezhkin; Z Fetisova
Journal:  Biophys J       Date:  1999-07       Impact factor: 4.033

Review 2.  How photosynthetic bacteria harvest solar energy.

Authors:  R J Cogdell; N W Isaacs; T D Howard; K McLuskey; N J Fraser; S M Prince
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

3.  The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX.

Authors:  R N Frese; J D Olsen; R Branvall; W H Westerhuis; C N Hunter; R van Grondelle
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

4.  Supramolecular complexes in photosynthetic bacteria.

Authors:  P A Loach
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

5.  The 7.5-A electron density and spectroscopic properties of a novel low-light B800 LH2 from Rhodopseudomonas palustris.

Authors:  Nichola Hartigan; Hazel A Tharia; Frank Sweeney; Anna M Lawless; Miroslav Z Papiz
Journal:  Biophys J       Date:  2002-02       Impact factor: 4.033

6.  Effect of carotenoids on the interaction between pigment-protein complexes in membranes of the sulfur photosynthetic bacterium Chromatium minutissimum.

Authors:  A A Moskalenko; O A Toropygina; Z A Zhuravleva; Y Erokhin
Journal:  Dokl Biochem Biophys       Date:  2001 Nov-Dec       Impact factor: 0.788

7.  Absorption and CD spectroscopy and modeling of various LH2 complexes from purple bacteria.

Authors:  Sofia Georgakopoulou; Raoul N Frese; Evelyn Johnson; Corline Koolhaas; Richard J Cogdell; Rienk van Grondelle; Gert van der Zwan
Journal:  Biophys J       Date:  2002-04       Impact factor: 4.033

8.  Temporally and spectrally resolved subpicosecond energy transfer within the peripheral antenna complex (LH2) and from LH2 to the core antenna complex in photosynthetic purple bacteria.

Authors:  S Hess; M Chachisvilis; K Timpmann; M R Jones; G J Fowler; C N Hunter; V Sundström
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-19       Impact factor: 11.205

9.  Significance of a two-domain structure in subunits of phycobiliproteins revealed by the normal mode analysis.

Authors:  H Kikuchi; H Wako; K Yura; M Go; M Mimuro
Journal:  Biophys J       Date:  2000-09       Impact factor: 4.033

10.  Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX.

Authors:  C Alistair Siebert; Pu Qian; Dimitrios Fotiadis; Andreas Engel; C Neil Hunter; Per A Bullough
Journal:  EMBO J       Date:  2004-02-05       Impact factor: 11.598
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