ADP controls the electrogenicity of Na/Na exchange catalyzed by dog kidney Na,K-ATPase proteoliposomes.

Sodium pump mediated Na/Na exchange was studied using Na(+)-loaded proteoliposomes prepared from dog kidney Na,K-ATPase. Measurements of both 22Na+ influx and pump-generated electrical potentials were carried out, the latter using the anionic dye, oxonol VI. In the presence of ATP, the formation of a strophanthidin-sensitive membrane potential confirms that Na/Na exchange associated with ATP hydrolysis can be electrogenic depending on the source of the enzyme. With the addition of varying concentrations of ADP, electrogenic exchange is progressively inhibited and replaced by electroneutral exchange. ADP is equally effective in activating (ATP + ADP)-dependent electroneutral exchange. With sufficient ADP, electrogenic Na/Na exchange is completely replaced by electroneutral exchange.