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Literature DB >> 787986

DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.

F Chu, H F Kung, P Caldwell, H Weissbach, N Brot.

Abstract

The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.

Entities: Chemical Species

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Year: 1976 PMID： 787986 PMCID： PMC430964 DOI： 10.1073/pnas.73.9.3156

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.

Authors: P S LEBOY; E C COX; J G FLAKS
Journal: Proc Natl Acad Sci U S A Date: 1964-12 Impact factor: 11.205

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Structural and functional evidence for a repeated 50S subunit ribosomal protein.

Authors: E Deusser; J Weber; R Maschler; G Stöffler; H G Wittmann
Journal: Nat New Biol Date: 1973-03-14

4. Stimulation of lac transcription by guanosine 5'-diphosphate 2'(or 3')-diphosphate and transfer ribonucleic acid.

Authors: M Aboud; I Pastan
Journal: J Biol Chem Date: 1973-05-10 Impact factor: 5.157

5. Further studies on the role of 50 S ribosomal proteins in protein synthesis.

Authors: N Brot; R Marcel; E Yamasaki; H Weissbach
Journal: J Biol Chem Date: 1973-10-25 Impact factor: 5.157

6. Studies on the in vitro transcription and translation of the lac operon.

Authors: H F Kung; N Brot; C Spears; B Chen; H Weissbach
Journal: Arch Biochem Biophys Date: 1974-01 Impact factor: 4.013

7. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.

Authors: S R Jaskunas; L Lindahl; M Nomura
Journal: Nature Date: 1975-10-09 Impact factor: 49.962

8. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.

Authors: L Lindahl; S R Jaskunas; P P Dennis; M Nomura
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

9. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.

Authors: J B Kirschbaum; E B Konrad
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.

1. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.

2. Protein measurement with the Folin phenol reagent.

3. Structural and functional evidence for a repeated 50S subunit ribosomal protein.

4. Stimulation of lac transcription by guanosine 5'-diphosphate 2'(or 3')-diphosphate and transfer ribonucleic acid.

5. Further studies on the role of 50 S ribosomal proteins in protein synthesis.

6. Studies on the in vitro transcription and translation of the lac operon.

7. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli.

8. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.

9. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.

10. Stringent control of ribosomal protein gene expression in Escherichia coli.

1. In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.

2. In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.

3. mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.

Review 4. Chemistry and biology of E. coli ribosomal protein L12.