Literature DB >> 787724

Preparation and counts of particles in electron microscopy: application of negative stain in the agarfiltration method.

E Kellenberger, D Bitterli.   

Abstract

The agarfiltration method, published by Kellenberger and Arber in 1957, has been adapted to negative stain with sodium phosphotungstate and uranyl acetate. The technique is described in detail including all recent improvements. The precision obtainable in particle counts is discussed for agarfiltration and sprayed droplets.

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Year:  1976        PMID： 787724

Source DB:  PubMed          Journal:  Microsc Acta        ISSN： 0044-376X


  9 in total

1.  Deletions in the transmembrane domain of a sindbis virus glycoprotein alter virus infectivity, stability, and host range.

Authors:  Raquel Hernandez; Christine Sinodis; Michelle Horton; Davis Ferreira; Chunning Yang; Dennis T Brown
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103

Review 2.  The bacterial nucleoid revisited.

Authors:  C Robinow; E Kellenberger
Journal:  Microbiol Rev       Date:  1994-06

3.  Plaque forming specialized transducing phage P1: isolation of P1CmSmSu, a precursor of P1Cm.

Authors:  S Iida; W Arber
Journal:  Mol Gen Genet       Date:  1977-06-24

4.  Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth.

Authors:  C L Woldringh; M A de Jong; W van den Berg; L Koppes
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

5.  Head maturation pathway of bacteriophages T4 and T2. III. Isolation and characterization of particles produced by mutants in gene 17.

Authors:  J L Carrascosa; E Kellenberger
Journal:  J Virol       Date:  1978-03       Impact factor: 5.103

6.  Head maturation pathway of bacteriophages T4 and T2. V. Maturable epsilon-particle accumulating an acridine-treated bacteriophage T4-infected cells.

Authors:  C Schaerli
Journal:  J Virol       Date:  1980-02       Impact factor: 5.103

7.  Head maturation pathway of bacteriophages T4 and T2. IV. In vitro transformation of T4 head-related particles produced by mutants in gene 17 to capsid-like structures.

Authors:  J L Carrascosa
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

8.  Chromosome replication rate and cell shape in Escherichia coli: lack of coupling.

Authors:  A Zaritsky; C L Woldringh
Journal:  J Bacteriol       Date:  1978-08       Impact factor: 3.490

Review 9.  Diagnostic electron microscopy is still a timely and rewarding method.

Authors:  S S Biel; H R Gelderblom
Journal:  J Clin Virol       Date:  1999-06       Impact factor: 3.168
  9 in total

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