Literature DB >> 7877186

Experimental study on anti-tumor effect of splenocytes induced by anti-CD3 McAb, PHA and IL-2.

G X Shen1, X L Wang, H F Zhu, Y Zhang, J F Shao.   

Abstract

The proliferation of splenocytes from healthy adults was induced by anti-CD3 McAb, PHA and IL-2. The proliferative capability and anti-tumor activity as well as phenotypes of the splenocytes cultured in different medium systems were studied. The results showed that anti-CD3 McAb and PHA not only enhanced the proliferation of splenocytes induced by IL-2, but also produced synergism if used simultaneously. The expressions of CD4 and Tac of cellular surface markers were increased after splenocytes were induced by anti-CD3 McAb and PHA. The results of anti-tumor activity of LAK cells suggested that PHA had the capability to promote anti-tumor activity of LAK cells by both direct and indirect pathways, but anti-CD3 McAb indirectly promoted anti-tumor activity of LAK cells by enhancing splenocyte proliferation.

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Year:  1994        PMID： 7877186     DOI： 10.1007/bf02888050

Source DB:  PubMed          Journal:  J Tongji Med Univ        ISSN： 0257-716X


  7 in total

1.  The importance of splenectomy for the adoptive immunotherapy of cancer.

Authors:  R E Schwarz; J C Hiserodt
Journal:  Med Hypotheses       Date:  1989-03       Impact factor: 1.538

2.  Long-term growth of human T cell lines and clones on anti-CD3 antibody-treated tissue culture plates.

Authors:  F C Garbrecht; C Russo; M E Weksler
Journal:  J Immunol Methods       Date:  1988-02-24       Impact factor: 2.303

3.  Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentrations of the T cell antigen receptor with either Lyt-2 or L3T4.

Authors:  K Eichmann; J I Jönsson; I Falk; F Emmrich
Journal:  Eur J Immunol       Date:  1987-05       Impact factor: 5.532

4.  Monoclonal antibodies directed to different epitopes in the CD3-TCR complex induce different states of competence in resting human T cells.

Authors:  R Schwinzer; R A Franklin; J Domenico; H Renz; E W Gelfand
Journal:  J Immunol       Date:  1992-03-01       Impact factor: 5.422

5.  Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells.

Authors:  M Londei; B Grubeck-Loebenstein; P de Berardinis; C Greenall; M Feldmann
Journal:  Scand J Immunol       Date:  1988-01       Impact factor: 3.487

6.  Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes.

Authors:  E A Grimm; A Mazumder; H Z Zhang; S A Rosenberg
Journal:  J Exp Med       Date:  1982-06-01       Impact factor: 14.307

7.  Regression of established pulmonary metastases and subcutaneous tumor mediated by the systemic administration of high-dose recombinant interleukin 2.

Authors:  S A Rosenberg; J J Mulé; P J Spiess; C M Reichert; S L Schwarz
Journal:  J Exp Med       Date:  1985-05-01       Impact factor: 14.307
  7 in total

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