Literature DB >> 7874769

Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.

E Appel1, S Eisenberg, J Roitelman.   

Abstract

We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.

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Year:  1995        PMID： 7874769

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  2 in total

Review 1.  Apolipoprotein E genotype, cardiovascular biomarkers and risk of stroke: systematic review and meta-analysis of 14,015 stroke cases and pooled analysis of primary biomarker data from up to 60,883 individuals.

Authors:  Tauseef A Khan; Tina Shah; David Prieto; Weili Zhang; Jackie Price; Gerald R Fowkes; Jackie Cooper; Philippa J Talmud; Steve E Humphries; Johan Sundstrom; Jaroslav A Hubacek; Shah Ebrahim; Debbie A Lawlor; Yoav Ben-Shlomo; Mohammad R Abdollahi; Arjen J C Slooter; Zoltan Szolnoki; Manjinder Sandhu; Nicholas Wareham; Ruth Frikke-Schmidt; Anne Tybjærg-Hansen; Gerda Fillenbaum; Bastiaan T Heijmans; Tomohiro Katsuya; Grazyna Gromadzka; Andrew Singleton; Luigi Ferrucci; John Hardy; Bradford Worrall; Stephen S Rich; Mar Matarin; John Whittaker; Tom R Gaunt; Peter Whincup; Richard Morris; John Deanfield; Ann Donald; George Davey Smith; Mika Kivimaki; Meena Kumari; Liam Smeeth; Kay-Tee Khaw; Michael Nalls; James Meschia; Kai Sun; Rutai Hui; Ian Day; Aroon D Hingorani; Juan P Casas
Journal:  Int J Epidemiol       Date:  2013-04       Impact factor: 7.196

2.  Apolipoprotein Gene Polymorphisms (APOB, APOC111, APOE) in the Development of Coronary Heart Disease in Ethnic Groups of Kazakhstan.

Authors:  S Berkinbayev; M Rysuly; A Mussayev; K Blum; N Baitasova; A Mussagaliyeva; G Dzhunusbekova; B Makhatov; Aa Mussayev; A Yeshmanova; R Lesbekova; Y Marchuk; R Azhibekova; M Oscar-Berman; M Kulmaganbetov
Journal:  J Genet Syndr Gene Ther       Date:  2014-01-24
  2 in total

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