| Literature DB >> 7874396 |
L R Sharer1, Y Saito, L G Epstein, B M Blumberg.
Abstract
In order to detect latent infection in neurons or other cell types in formalin-fixed brain tissue, we performed polymerase chain reaction amplification with incorporation of digoxigenin-conjugated deoxynucleotides, followed by in situ hybridization with biotinylated probes. The use of this two-step technique in brain tissue from a child with severe HIV-1 encephalitis revealed signal in both nuclear and perinuclear regions of cells identified as monocytes and astrocytes, and also in perineuronal satellite cells of glial morphology, but HIV-1 infection of neurons was not detected.Entities:
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Year: 1994 PMID： 7874396 DOI： 10.1016/s0960-5428(06)80268-8
Source DB: PubMed Journal: Adv Neuroimmunol ISSN： 0960-5428