Literature DB >> 7873783

AVRXa10 protein is in the cytoplasm of Xanthomonas oryzae pv. oryzae.

S A Young1, F F White, C M Hopkins, J E Leach.   

Abstract

AVRXa10 from Xanthomonas oryzae pv. oryzae was tagged with a unique hydrophilic octapeptide (FLAG) to permit antibody-mediated identification and purification of the gene product. X. o. pv. oryzae that produced tagged AVRXa10 elicited a hypersensitive response (HR) on rice cultivars containing the resistance gene Xa-10, but not on cultivars lacking Xa-10. The tagged AVRXa10 protein purified from Escherichia coli or X. o. pv. oryzae did not elicit a hypersensitive response in rice with the Xa-10 resistance gene. Anti-FLAG monoclonal antibodies reacted with a 119-kDa protein in both E. coli and X. o. pv. oryzae cells expressing the tagged avrXa10 gene. Polyclonal antibodies raised against purified AVRXa10 protein reacted with the 119-kDa protein and several additional proteins from X. o. pv. oryzae, which probably are the products of genes related to avrXa10. Biochemical fractionation and immunoelectronmicroscopy analysis was used to demonstrate that AVRXa10 was located in the cytoplasm of X. o. pv. oryzae cells when grown in planta or in culture medium.

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Year:  1994        PMID： 7873783     DOI： 10.1094/mpmi-7-0799

Source DB:  PubMed          Journal:  Mol Plant Microbe Interact        ISSN： 0894-0282            Impact factor:   4.171


  8 in total

1.  The C terminus of AvrXa10 can be replaced by the transcriptional activation domain of VP16 from the herpes simplex virus.

Authors:  W Zhu; B Yang; N Wills; L B Johnson; F F White
Journal:  Plant Cell       Date:  1999-09       Impact factor: 11.277

2.  Bacterial Pathogens in Plants: Life up against the Wall.

Authors:  J. R. Alfano; A. Collmer
Journal:  Plant Cell       Date:  1996-10       Impact factor: 11.277

3.  Erwinia amylovora secretes DspE, a pathogenicity factor and functional AvrE homolog, through the Hrp (type III secretion) pathway.

Authors:  A J Bogdanove; D W Bauer; S V Beer
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

Review 4.  The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and death.

Authors:  J R Alfano; A Collmer
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

5.  Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death.

Authors:  S Gopalan; D W Bauer; J R Alfano; A O Loniello; S Y He; A Collmer
Journal:  Plant Cell       Date:  1996-07       Impact factor: 11.277

6.  Changes in the Plasma Membrane Distribution of Rice Phospholipase D during Resistant Interactions with Xanthomonas oryzae pv oryzae.

Authors:  S. A. Young; X. Wang; J. E. Leach
Journal:  Plant Cell       Date:  1996-06       Impact factor: 11.277

7.  A cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture.

Authors:  J H Ham; D W Bauer; D E Fouts; A Collmer
Journal:  Proc Natl Acad Sci U S A       Date:  1998-08-18       Impact factor: 11.205

8.  Rice cationic peroxidase accumulates in xylem vessels during incompatible interactions with Xanthomonas oryzae pv oryzae.

Authors:  S A Young; A Guo; J A Guikema; F F White; J E Leach
Journal:  Plant Physiol       Date:  1995-04       Impact factor: 8.340
  8 in total

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