Literature DB >> 78735

Hydrodynamic orientation of sperm heads for flow cytometry.

P N Dean, D Pinkel, M L Mendelsohn.   

Abstract

Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.

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Year:  1978        PMID： 78735      PMCID： PMC1473544          DOI： 10.1016/S0006-3495(78)85428-9

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  8 in total

1.  Basic principles of electrical sizing of cells and particles and their realization in the new instrument "Metricell".

Authors:  V Kachel
Journal:  J Histochem Cytochem       Date:  1976-01       Impact factor: 2.479

2.  A means for orienting flat cells in flow systems.

Authors:  R T Stovel; R G Sweet; L A Herzenberg
Journal:  Biophys J       Date:  1978-07       Impact factor: 4.033

3.  Hydrodynamic orientation of cells.

Authors:  M J Fulwyler
Journal:  J Histochem Cytochem       Date:  1977-07       Impact factor: 2.479

4.  Experimental findings on gynecologic cell orientation and dynamics for three flow nozzle geometries.

Authors:  D B Kay; L L Wheeless
Journal:  J Histochem Cytochem       Date:  1977-07       Impact factor: 2.479

5.  Uniform lateral orientation, caused by flow forces, of flat particles in flow-through systems.

Authors:  V Kachel; E Kordwig; E Glossner
Journal:  J Histochem Cytochem       Date:  1977-07       Impact factor: 2.479

6.  Cell microfluorometry: a method for rapid fluorescence measurement.

Authors:  M A Van Dilla; T T Trujillo; P F Mullaney; J R Coulter
Journal:  Science       Date:  1969-03-14       Impact factor: 47.728

7.  Flow microfluorometric analysis of sperm DNA content: effect of cell shape on the fluorescence distribution.

Authors:  B L Gledhill; S Lake; L L Steinmetz; J W Gray; P N Dean; M A Van Dilla
Journal:  J Cell Physiol       Date:  1976-03       Impact factor: 6.384

8.  Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches.

Authors:  M A Van Dilla; B L Gledhill; S Lake; P N Dean; J W Gray; V Kachel; B Barlogie; W Göhde
Journal:  J Histochem Cytochem       Date:  1977-07       Impact factor: 2.479
  8 in total
  2 in total

1.  Flow cytometry of human spermatozoa.

Authors:  F J Otto; U Hacker; J Zante; J Schumann; W Göhde; M L Meistrich
Journal:  Histochemistry       Date:  1979-07-11

2.  Characteristics of a simple, high-resolution flow cytometer based o a new flow configuration.

Authors:  T Lindmo; H B Steen
Journal:  Biophys J       Date:  1979-10       Impact factor: 4.033
  2 in total

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